Human CD44 ELISA Set (ab212086)
- Datasheet
- References
- Protocols
Overview
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Product name
Human CD44 ELISA Set
See all CD44 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.13 ng/ml -
Range
10 ng/ml - 0.31 ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s CD44 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) Set is designed for the quantitative measurement of soluble CD44 in Human serum, plasma, buffered solutions and cell culture medium.
The ELISA set contains 100 µg of unlabeled, mouse monoclonal capture antibody and 50 µg of mouse monoclonal detector antibody. Lyophilized Human CD44 protein purified from Human serum is included for quantification. The exact concentration of the protein standard can be found on the vials’ label. Detection requires an secondary antibody labeled with biotin.
The protocol booklet contains guidelines on how to use the ELISA Set for a sandwich ELISA with a secondary antibody for detection.
This kit will recognize both endogenous and recombinant Human CD44.
Optimization of the ELISA Set reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this ELISA Set in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Optimization of the ELISA Set reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this ELISA Set in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Additional protocol information and tip on the use of the Human CD44 ELISA Set for sandwich ELISA can be found on our website:
https://www.abcam.com/kits/how-to-use-matched-antibody-pair-kits-for-sandwich-elisa
For additional information on the performance of the antibody pair used in this ELISA Set, please see our equivalent CD44 Human ELISA Set (ab45912) which uses the same antibody pair.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 100 tests Human CD44 Capture Antibody 1 x 100µg Biotinylated anti-CD44 anitibody Red 1 x 50µg Human CD44 Standard Protein 1 x 139ng -
Research areas
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- LHR
- BA-1
- CD 44
see all -
Database links
- Entrez Gene: 960 Human
- Omim: 107269 Human
- SwissProt: P16070 Human
- Unigene: 502328 Human
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab212086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Dose Response Curve (Logarithmic)
Protocols
References
ab212086 has not yet been referenced specifically in any publications.
