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    human-cd44-elisa-set-ab212086.pdf

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Immunology Cell Type Markers CD Adhesion
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Human CD44 ELISA Set (ab212086)

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  • SDS
  • Protocol Booklet
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Dose Response Curve (Logarithmic)

    Key features and details

    • Sensitivity: 0.13 ng/ml
    • Range: 10 ng/ml - 0.31 ng/ml
    • Sample type: Cell culture supernatant, Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Protein
    Product image
    Recombinant human CD44 protein (Active) (ab173996)

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    Overview

    • Product name

      Human CD44 ELISA Set
      See all CD44 kits
    • Detection method

      Colorimetric
    • Sample type

      Cell culture supernatant, Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      0.13 ng/ml
    • Range

      10 ng/ml - 0.31 ng/ml
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s CD44 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) Set is designed for the quantitative measurement of soluble CD44 in Human serum, plasma, buffered solutions and cell culture medium.


      The ELISA set contains 100 µg of unlabeled, mouse monoclonal capture antibody and 50 µg of mouse monoclonal detector antibody. Lyophilized Human CD44 protein purified from Human serum is included for quantification. The exact concentration of the protein standard can be found on the vials’ label. Detection requires an  secondary antibody labeled with biotin.


      The protocol booklet contains guidelines on how to use the ELISA Set for a sandwich ELISA with a secondary antibody for detection.


      This kit will recognize both endogenous and recombinant Human CD44.


      Optimization of the ELISA Set reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this ELISA Set in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


      Optimization of the ELISA Set reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this ELISA Set in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


      Additional protocol information and tip on the use of the Human CD44 ELISA Set for sandwich ELISA can be found on our website:


       https://www.abcam.com/kits/how-to-use-matched-antibody-pair-kits-for-sandwich-elisa 


      For additional information on the performance of the antibody pair used in this ELISA Set, please see our equivalent CD44 Human ELISA Set (ab45912) which uses the same antibody pair.

    • Platform

      Reagents

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components Identifier 100 tests
      Human CD44 Capture Antibody 1 x 100µg
      Biotinylated anti-CD44 anitibody Red 1 x 50µg
      Human CD44 Standard Protein 1 x 139ng
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion
      • Stem Cells
      • Mesenchymal Stem Cells
      • Surface Molecules
      • Cancer
      • Tumor immunology
      • CD markers
      • Cancer
      • Tumor biomarkers
      • Other
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Neutrophil Lineage
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • CD markers ELISA kits
    • Function

      Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.
    • Tissue specificity

      Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.
    • Sequence similarities

      Contains 1 Link domain.
    • Domain

      The lectin-like LINK domain is responsible for hyaluronan binding.
    • Post-translational
      modifications

      Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
      N-glycosylated.
      O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
      Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P16070 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • LHR
      • BA-1
      • CD 44
      • CD44
      • CD44 antigen
      • CD44 molecule
      • CD44 molecule (Indian blood group)
      • CD44_HUMAN
      • CDw44
      • CDW44 antigen
      • Cell surface glycoprotein CD44
      • chondroitin sulfate proteoglycan 8
      • CSPG8
      • ECMR-III
      • Epican
      • Extracellular matrix receptor III
      • GP90 lymphocyte homing/adhesion receptor
      • HCELL
      • hematopoietic cell E- and L-selectin ligand
      • Heparan sulfate proteoglycan
      • Hermes antigen
      • homing function and Indian blood group system
      • HSA
      • HUTCH-I
      • HUTCH1
      • HUTCHI
      • Hyaluronate receptor
      • IN
      • INLU-related p80 Glycoprotein
      • MC56
      • MDU2
      • MDU3
      • MGC10468
      • MIC4
      • MUTCH I
      • MUTCH1
      • PGP-1
      • PGP-I
      • PGP1
      • Phagocytic glycoprotein 1
      • Phagocytic glycoprotein I
      • Soluble CD44
      see all
    • Database links

      • Entrez Gene: 960 Human
      • Omim: 107269 Human
      • SwissProt: P16070 Human
      • Unigene: 502328 Human

      Associated products

      • Related Products

        • Human CD44 ELISA Kit (ab45912)

      Images

      • Dose Response Curve (Logarithmic)
        Dose Response Curve (Logarithmic)

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab212086? Please let us know so that we can cite the reference in this datasheet.

      ab212086 has not yet been referenced specifically in any publications.

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