Overview

  • Product name

    Human CD48 ELISA kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 1380pg/ml 2.4%
    2 16 420pg/ml 3.2%
    3 16 170pg/ml 2.9%
    4 16 100pg/ml 4.2%
    Inter-assay
    Sample n Mean SD CV%
    1 3 1293pg/ml 6.97%
    2 3 410pg/ml 2.44%
    3 3 173pg/ml 3.33%
    4 3 100pg/ml 0%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    2 pg/ml
  • Range

    25 pg/ml - 1600 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Spike 107.33 25pg/ml - 1600pg/ml

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse
  • Product overview

    The human CD48 ELISA kit (ab215419) is deigned for the quantitative determination of human CD48 in cell culture supernatant, serum and plasma.


     

  • Notes

    The human CD48 test is based on the quantitative sandwich enzyme immunoassay technique. Microplate wells are pre-coated with anti-human CD48-specific polyclonal capture antibodies. Samples and standards are pipetted into microwells and human CD48 molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human CD48-specific polyclonal detection antibodies bind to different epitopes of human CD48 molecules. After washing, the ready to use HRP substrate (TMB) is added to wells. The intensity of the color produced is directly proportional to the amount of human CD48 in the sample. Color development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.

     

     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Anti-Human CD48 coated microplate (12 x 8 wells) 1 unit
    Human CD48 sample diluent 1 x 25ml
    Human CD48 standard A (0 pg/mL) 1 x 1ml
    Human CD48 standard B (25 pg/mL) 1 x 1ml
    Human CD48 standard C (50 pg/mL) 1 x 1ml
    Human CD48 standard D (100 pg/mL) 1 x 1ml
    Human CD48 standard E (200 pg/mL) 1 x 1ml
    Human CD48 standard F (400 pg/mL) 1 x 1ml
    Human CD48 standard G (800 pg/mL) 1 x 1ml
    Human CD48 standard H (1600 pg/mL) 1 x 1ml
    Human CD48 enzyme conjugate stock 1 x 30µl
    Human CD48 enzyme conjugate stock diluent 1 x 12ml
    10X Wash concentrate 1 x 50ml
    TMB Substrate solution 1 x 12ml
    Stop solution 1 x 12ml
  • Research areas

  • Function

    Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Alternative names

    • Antigen CD48
    • B cell membrane protein
    • B lymphocyte activation marker BLAST 1
    • B-cell activation marker
    • B-lymphocyte activation marker BLAST-1
    • BCM 1 surface antigen
    • BCM1
    • BCM1 surface antigen
    • BLAST
    • BLAST 1
    • BLAST1
    • CD 48
    • CD48
    • CD48 antigen
    • CD48 antigen (B cell membrane protein)
    • CD48 molecule
    • CD48 protein
    • CD48_HUMAN
    • hCD48
    • Leucocyte antigen MEM 102
    • Leukocyte antigen MEM-102
    • mCD48
    • MEM 102
    • MEM-102
    • MEM102
    • Signaling lymphocytic activation molecule 2
    • SLAM family member 2
    • SLAMF 2
    • SLAMF2
    • TCT.1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab215419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Cubic spline fit curve on a log-log scale.

  • Linear regression (LLS) fit curve on a lin-lin scale.

Protocols

References

ab215419 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215419.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up