Key features and details
- One-wash 90 minute protocol
- Sensitivity: 13.8 pg/ml
- Range: 47 pg/ml - 3000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman CD51 ELISA Kit
See all Integrin alpha V kits
Intra-assay Sample n Mean SD CV% Tissue 8 6.4%
Sample typeCell culture extracts, Tissue Extracts
Assay typeSandwich (quantitative)
Range47 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture extracts 90 % - % Tissue Extracts 94 % - %
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Human
Human CD51 ELISA Kit (ab235650) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD51 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD51 with 13.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Human CD51 Capture Antibody 1 x 600µl 10X Human CD51 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human CD51 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionThe alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similaritiesBelongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
- Information by UniProt
- antigen identified by monoclonal antibody L230
- CD 51
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
Interpolated concentrations of native CD51 in HT-29 cell extract, human brain extract, and NIH3T3 Pervanadate-treated extract samples based on a 500 μg/mL, 250 μg/mL, and 62.5 μg/mL extract load, respectively. The concentrations of CD51 were measured in duplicate and interpolated from the CD51 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD51 concentration was determined to be 1.52 ng/mL in HT-29 cell extract, 1.47 ng/mL in human brain extract and 0.87 ng/mL in NIH3T3 Pervanadate-treated extract.
To learn more about the advantages of recombinant antibodies see here.
ab235650 has been referenced in 1 publication.
- Iosef C et al. Quantitative proteomics reveal lineage-specific protein profiles in iPSC-derived Marfan syndrome smooth muscle cells. Sci Rep 10:20392 (2020). PubMed: 33230159