Human CD5L / CT-2 ELISA Kit (ab213760)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD5L / CT-2 ELISA Kit
See all CD5L/CT-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 245pg/ml 10.04 4.1% 2 16 1172pg/ml 79.69 6.8% 3 16 5450pg/ml 414.2 7.6% Inter-assay Sample n Mean SD CV% 1 24 224pg/ml 13.21 5.9% 2 24 1288pg/ml 91.44 7.1% 3 24 5192pg/ml 415.36 8% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human CD5L / CT-2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213760) is designed for the quantitative measurement of Human CD5L / CT-2 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD5L / CT-2 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S20-G347) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD5L / CT-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD5L / CT-2 amount of sample captured in plate.
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Notes
CD5 antigen-like, also known as Sp alpha and AIM, is a protein that in humans is encoded by the CD5L gene. It is mapped to 1q21-q23 by fluorescence in situ hybridization. It is found that Aim expression is induced in mouse macrophages in response to loading with highly oxidized low density lipoprotein (oxLDL), and that Aim is expressed in foam cells within atherosclerotic lesions. Both the expression of Aim in lesions and its induction by oxLDL require Lxr /Rxr heterodimers. Aim-null macrophages are highly susceptible to oxLDL-induced apoptosis in vitro and undergo accelerated apoptosis in atherosclerotic lesions in vivo. Double knockout of Aim and Ldlr reduce atherosclerotic lesions. Therefore, it is concluded that AIM expression protects macrophages from apoptosis within atherosclerotic lesions, promoting early lesion development.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human CD5L coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human CD5L antibody 1 x 100µl Lyophilized recombinant Human CD5L standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
May play a role in the regulation of the immune system. Seems to play a role as an inhibitor of apoptosis. -
Tissue specificity
Expressed in spleen, lymph node, thymus, bone marrow, and fetal liver, but not in non-lymphoid tissues. -
Sequence similarities
Contains 3 SRCR domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AAC-11
- AIM
- API6
see all -
Database links
- Entrez Gene: 922 Human
- Omim: 602592 Human
- SwissProt: O43866 Human
- Unigene: 134035 Human
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab213760 has been referenced in 1 publication.
- Wang C et al. Proteomic Analysis of the Alterations in Follicular Fluid Proteins During Oocyte Maturation in Humans. Front Endocrinol (Lausanne) 12:830691 (2021). PubMed: 35185790