Key features and details
- Sensitivity: 30 pg/ml
- Range: 24.69 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman CD62E ELISA Kit (Selectin)
See all CD62E kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 30 pg/ml
Range24.69 pg/ml - 18000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.39 83% - 103% Serum 93.75 83% - 102% Plasma 95.38 84% - 105%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s CD62E (Selectin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62E in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CD62E coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 900x HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human CD62E 2 vials CD62E Microplate (12 strips x 8 wells) 1 unit Recombinant Human CD62E Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionCell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
Sequence similaritiesBelongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains.
- Information by UniProt
- CD62 antigen-like family member E
ab100512 has been referenced in 1 publication.
- Chitalia N et al. Impact of vitamin D supplementation on arterial vasomotion, stiffness and endothelial biomarkers in chronic kidney disease patients. PLoS One 9:e91363 (2014). PubMed: 24646518