• Product name
    Human CD62P ELISA Kit (P-Selectin)
    See all CD62P kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 20 pg/ml
  • Range
    0.041 ng/ml - 30 ng/ml
  • Recovery

    72 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 72.51 63% - 85%
    Serum 74.98 65% - 86%
    Plasma 68.29 60% - 78%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD62P (P-Selectin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62P in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CD62P coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62P present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62P antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62P bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    800X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human CD62P (lyophilized) 2 vials
    CD62P Microplate (12 x 8 wells) 1 unit
    Recombinant Human CD62P Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • Tissue specificity
    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • Involvement in disease
    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similarities
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • Cellular localization
  • Information by UniProt
  • Alternative names
    • Antigen CD62
    • CD62
    • CD62 antigen like family member P
    • CD62 antigen-like family member P
    • CD62P
    • FLJ45155
    • GMP 140
    • GMP-140
    • GMP140
    • Granule membrane protein 140
    • Granule membrane protein 140kDa
    • Granulocyte membrane protein
    • GRMP
    • LECAM3
    • Leukocyte endothelial cell adhesion molecule 3
    • Leukocyte-endothelial cell adhesion molecule 3
    • P selectin
    • P-selectin
    • PADGEM
    • Platelet activation dependent granule external membrane protein
    • Platelet activation dependent granule-external membrane protein
    • Platelet alpha granule membrane protein
    • PSEL
    • Selectin P
    • Selectin P (granule membrane protein 140kDa antigen CD62)
    • SELP
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100631.

  • Representative Standard Curve using ab100631.



This product has been referenced in:
  • Ghasemzadeh M  et al. Reactive Oxygen Species Generated by CD45-Cells Distinct from Leukocyte Population in Platelet Concentrates Is Correlated with the Expression and Release of Platelet Activation Markers during Storage. Transfus Med Hemother 45:33-41 (2018). Read more (PubMed: 29593458) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your reply. I agree with your points. The protocol did not specify which diluent to use and this is likely to be the cause of the problem. I was hoping that you might have some reagents left to try the procedure again with the changes ...

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Thank you for your patience. I received further information from the lab regarding component dilution. - Briefly spin the HRP-Streptavidin concentrate vial and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be...

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I am very sorry for the delay. I received the following information from the lab.Please note that diluent B should be used to dilute HRP-streptavidin: - Detection Antibody – once reconstituted, this must be used within a couple of days. We provi...

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Thank you for your patience. I have just received this reply for the lab, would it be possible for you to answer their questions? (1) After reviewing the data I noticed there were 90 samples and 6 standards. The sample identifications just say ...

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Thank you for your patience. The lab have requested some additional information to investigate the problem in more detail. How was the kit stored and for how long? Type of sample, if diluted what dilution was used? How were the samples prepared...

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