Key features and details
- Sensitivity: 20 pg/ml
- Range: 0.041 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman CD62P ELISA Kit (P-Selectin)
See all CD62P kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 20 pg/ml
Range0.041 ng/ml - 30 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 72.51 63% - 85% Serum 74.98 65% - 86% Plasma 68.29 60% - 78%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s CD62P (P-Selectin) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62P in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CD62P coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62P present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62P antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62P bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human CD62P (lyophilized) 2 vials CD62P Microplate (12 x 8 wells) 1 unit Recombinant Human CD62P Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionCa(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
Tissue specificityStored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Involvement in diseaseDefects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Sequence similaritiesBelongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 9 Sushi (CCP/SCR) domains.
- Information by UniProt
- Antigen CD62
- CD62 antigen like family member P
ab100631 has been referenced in 2 publications.
- Ghasemzadeh M et al. Reactive Oxygen Species Generated by CD45-Cells Distinct from Leukocyte Population in Platelet Concentrates Is Correlated with the Expression and Release of Platelet Activation Markers during Storage. Transfus Med Hemother 45:33-41 (2018). PubMed: 29593458
- Alsmadi NZ et al. Constricted microfluidic devices to study the effects of transient high shear exposure on platelets. Biomicrofluidics 11:064105 (2017). PubMed: 29204246