Overview

  • Product name

    Human CD86 ELISA Kit
    See all CD86 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample A 8 37.25U/ml 0.329 0.88%
    Sample B 8 5.09U/ml 0.193 3.79%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 39 37.63U/ml 0.611 1.62%
    Sample B 39 4.92U/ml 0.348 7.06%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.6 U/ml
  • Range

    1.187 U/ml - 38 U/ml
  • Assay time

    2h 40m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human CD86 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD86 in Human serum, plasma, buffered solutions or cell culture medium.

    A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD86 present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD86.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 2 x 96 tests 1 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 2 x 10ml 1 x 10ml
    Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7ml
    Biotinylated anti-CD86 Red 2 x 0.4ml 1 x 0.4ml
    CD86 Microplate (12 x 8 well strips) 2 units 1 unit
    CD86 Standard (Lyophilized) Yellow 4 vials 2 vials
    Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 2 x 11ml 1 x 11ml
    Streptavidin-HRP 4 x 5µl 2 x 5µl
  • Research areas

  • Function

    Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
  • Tissue specificity

    Expressed by activated B-lymphocytes and monocytes.
  • Sequence similarities

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Post-translational
    modifications

    Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • Activation B7-2 antigen
    • Activation B7-2 antigen 3
    • B-lymphocyte activation antigen B7-2
    • B-lymphocyte activation antigen B7-2 2
    • B7
    • B7 2
    • B7-2
    • B7.2
    • B70
    • B72
    • B72 antigen
    • BU63
    • CD28 antigen ligand 2
    • CD28 antigen ligand 2 2
    • Cd28l2
    • CD28LG2
    • CD86
    • CD86 antigen
    • CD86 antigen (CD28 antigen ligand 2 B7 2 antigen)
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
    • CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2
    • CD86 molecule
    • CD86_HUMAN
    • CLS1
    • CTLA-4 counter-receptor B7.2
    • CTLA-4 counter-receptor B7.2 2
    • CTLA-4 counter-receptor B7.2 2, 3
    • Early T-cell costimulatory molecule 1
    • ETC-1
    • FUN 1
    • FUN-1
    • FUN1
    • LAB72
    • Ly-58
    • Ly58
    • MB7
    • MB7-2
    • Membrane glycoprotein
    • MGC34413
    • T lymphocyte activation antigen CD86 precursor
    • T-lymphocyte activation antigen CD86
    • TS/A-2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab45921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • CD86 in U937 lysates from control cells or cells stimulated for 24 hours with 50 ng x mL-1 PMA (ab120297) or PMA with 1 ug x mL-1 LPS for the last 6 hours (duplicates; +/- SD).

    2e6 snap frozen cells were lysed in 0.3 mL RIPA lysis buffer on ice, sonicated in an ice cold water bath for 12 intervals for a total of 3.5 minutes. The supernatants were cleared by centrifugation (max speed, 10 minutes, 4 degrees), and tested undiluted or diluted 1/4.

  • CD86 measured in undiluted 72 hours culture supernatants from control of treated (5 ug x mL-1 PHA; Sigma) PBMCs (1e6 cells x mL-1; duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Representative Standard Curve using ab45921

Protocols

References

This product has been referenced in:

  • Smets I  et al. Multiple sclerosis risk variants alter expression of co-stimulatory genes in B cells. Brain N/A:N/A (2018). Sandwich ELISA . Read more (PubMed: 29361022) »
  • Ziliotto N  et al. Increased CCL18 plasma levels are associated with neurodegenerative MRI outcomes in multiple sclerosis patients. Mult Scler Relat Disord 25:37-42 (2018). Read more (PubMed: 30031282) »
See all 2 Publications for this product

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