Product nameHuman CD9 knockout A549 cell lysate
See all CD9 kits
Knockout cell lysate achieved by CRISPR/Cas9.
Parental Cell LineA549
Mutation descriptionKnockout achieved by CRISPR/Cas9; X = 7 bp deletion; 1 bp deletion; Frameshift = 100%
Knockout validationNext Generation Sequencing (NGS), Western Blot (WB)
Reconstitution notesTo use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
See here for more information on knockout cell lysates.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Tested applicationsSuitable for: WBmore details
Storage instructionsStore at -80°C. Please refer to protocols.
Components 1 kit ab280437 - Human CD9 knockout A549 cell lysate 1 x 100µg ab259782 - Human wild-type A549 cell lysate 1 x 100µg
FunctionInvolved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
Tissue specificityExpressed by a variety of hematopoietic and epithelial cells.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
- Information by UniProt
- Tetraspanin 29
- 5H9 antigen
KO cell lines
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab261687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use at an assay dependent concentration.
Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2: CD9 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lanes 1 - 3: Merged signal (red and green). Green - ab92726 observed at 22 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab92726 was shown to recognize CD9 in wild-type A549 cells as signal was lost at the expected MW in CD9 knockout cell line ab261878 (knockout cell lysate ab261687). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and CD9 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab92726 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Knockout achieved by CRISPR/Cas9; X = 7 bp deletion, 1 bp deletion; Frameshift = 100%
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab261687 has not yet been referenced specifically in any publications.