Overview

  • Product name

    Human CD93 ELISA Kit
    See all CD93 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 6.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.5%
  • Sample type

    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    13.4 pg/ml
  • Range

    70 pg/ml - 4500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 99 94% - 105%
    Urine 103 98% - 109%
    Serum 107 100% - 119%
    Heparin Plasma 100 99% - 100%
    EDTA Plasma 96 91% - 99%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    CD93 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD93 protein in human serum, plasma, cell culture supernatant, and urine samples. 


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm. 


    CD93 is a transmembrane glycoprotein involved in macrophage and monocyte phagocytosis and intracellular adhesion. CD93 is a receptor for MBL2 and SPA, and is highly expressed in myeloid-derived cells.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human CD93 Capture Antibody 1 x 600µl
    10X Human CD93 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Human CD93 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.
  • Tissue specificity

    Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 5 EGF-like domains.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • C1q receptor 1
    • C1q/MBL/SPA receptor
    • C1qR
    • C1qR(p)
    • C1qr1
    • C1QR1_HUMAN
    • C1qRp
    • CD93
    • CD93 antigen
    • CD93 molecule
    • CDw93
    • Complement component 1 q subcomponent receptor 1
    • Complement component C1q receptor
    • dJ737E23.1
    • ECSM3
    • Matrix remodeling associated protein 4
    • Matrix remodelling associated 4
    • Matrix-remodeling-associated protein 4
    • MXRA4
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab235636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between human CD93 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 2%, plasma (heparin) 2% and plasma (EDTA) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 191.2 ng/mL in serum, 189.7 ng/mL in plasma (citrate), 166.8 ng/ml in plasma (heparin), and 255.0 ng/mL in plasma (EDTA).

  • The concentrations of CD93 were measured in duplicates, interpolated from the CD93 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50% and LPS-treated THP-1 supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 1.2 ng/mL in urine, and 6.3 ng/ml in treated THP-1 supernatant.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD93 concentration was determined to be 230.4 ng/mL with a range of 134.8 – 719.6 ng/mL.

Protocols

References

ab235636 has not yet been referenced specifically in any publications.

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