Overview

  • Product name

    Human CD93 ELISA Kit (C1qR)
    See all CD93 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 634pg/ml 33.6 5.3%
    2 16 1493pg/ml 92.6 6.2%
    3 16 2436pg/ml 107.2 4.4%
    Inter-assay
    Sample n Mean SD CV%
    1 24 572pg/ml 38.9 6.8%
    2 24 1620pg/ml 121.5 7.5%
    3 24 2864pg/ml 160.4 5.6%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Heparin Plasma, EDTA Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    78 pg/ml - 5000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human CD93 Enzyme-Linked Immunosorbent Assay (ELISA) kit (C1qR) (ab213762) is designed for the quantitative measurement of Human CD93 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD93/C1qR has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A24-K580) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD93/C1qR is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD93/C1qR amount of sample captured in plate.

  • Notes

    CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the re-modeling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human CD93 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human CD93 antibody 1 x 130µl
    Lyophilized recombinant Human CD93 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.
  • Tissue specificity

    Highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. Not expressed in cells of lymphoid origin.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 5 EGF-like domains.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Alternative names

    • C1q receptor 1
    • C1q/MBL/SPA receptor
    • C1qR
    • C1qR(p)
    • C1qr1
    • C1QR1_HUMAN
    • C1qRp
    • CD93
    • CD93 antigen
    • CD93 molecule
    • CDw93
    • Complement component 1 q subcomponent receptor 1
    • Complement component C1q receptor
    • dJ737E23.1
    • ECSM3
    • Matrix remodeling associated protein 4
    • Matrix remodelling associated 4
    • Matrix-remodeling-associated protein 4
    • MXRA4
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human CD93 ELISA Kit (C1qR) (ab213762) Standard Curve.

Protocols

References

ab213762 has not yet been referenced specifically in any publications.

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