Overview

  • Product name
    Human CD95 ELISA Kit (TNFRSF6)
    See all Fas kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sample A 14 1719pg/ml 106 6.1%
    Sample B 16 703pg/ml 39 5.5%
    Inter-assay
    Sample n Mean SD CV%
    Sample A 8 1752pg/ml 145 8.2%
    Sample B 8 856pg/ml 68 7.9%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 47 pg/ml
  • Range
    93.75 pg/ml - 3000 pg/ml
  • Assay time
    1h 45m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human soluble CD95 (TNFRSF6) ELISA is to be used for the in-vitro quantitative determination of soluble CD95 in Human serum, plasma, buffered solutions or cell culture medium.


    A monoclonal antibody specific for CD95 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD95 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD95 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD95 present in the samples. 


    This kit will recognize both endogenous and recombinant Human CD95.


    Get results in 90 minutes with Human FASLG Receptor ELISA Kit (CD95) (ab183360) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-CD95 Red 1 x 0.4ml 2 x 0.4ml
    CD95 Control Silver 2 vials 4 vials
    CD95 Microplate (12 x 8 well strips) 1 unit 2 units
    CD95 Standard (Lyophilized) Yellow 2 vials 4 vials
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
  • Research areas
  • Function
    Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
  • Tissue specificity
    Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
  • Involvement in disease
    Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
  • Sequence similarities
    Contains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • Domain
    Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • ALPS 1A
    • ALPS1A
    • APO 1
    • Apo 1 antigen
    • APO 1 cell surface antigen
    • Apo-1 antigen
    • APO1
    • Apo1 antigen
    • APO1 cell surface antigen
    • Apoptosis antigen 1
    • Apoptosis mediating surface antigen FAS
    • Apoptosis-mediating surface antigen FAS
    • APT 1
    • APT1
    • CD 95
    • CD 95 antigen
    • CD95
    • CD95 antigen
    • Delta Fas
    • Delta Fas/APO 1/CD95
    • Delta Fas/APO1/CD95
    • Fas
    • Fas (TNF receptor superfamily, member 6)
    • FAS 1
    • FAS 827dupA
    • Fas AMA
    • FAS Antigen
    • Fas cell surface death receptor
    • FAS1
    • FASLG receptor
    • FASTM
    • sFAS
    • Surface antigen APO1
    • TNF receptor superfamily, member 6
    • TNFRSF 6
    • TNFRSF6
    • TNR6_HUMAN
    • Tumor necrosis factor receptor superfamily member 6
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab45922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab45922

Protocols

References

ab45922 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your enquiry.

I have reviewed the details on the datasheets for these kits. For the Fas ligand kits:

ab45892 Fas Ligand Human ELISA Kit
ab100515 Fas Ligand Human ELISA Kit

Sensitivities are different:ab100515 < 2 pg/ml, ab45892 < 12 pg/ml

Detection ranges are different:ab10515 1.37 pg/ml - 1000 pg/ml,ab45892 62.5 pg/ml - 2000 pg/ml

For the CD95 kits:
ab45922 CD95 Human ELISA Kit
ab100513 CD95 Human ELISA Kit

Again, sensitivities are different: ab100513 < 5 pg/ml, ab45922 < 47 pg/ml
Detection ranges are different:ab45922 93.75 pg/ml - 3000 pg/ml, ab100513 2.74 pg/ml - 2000 pg/ml

These are the main differences you may need to take into consideration. However, I can recommend to review the datasheets fullyfor further information and otherdifferences.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for your inquiry.

These ELISA kits are from two different sources but they are similar.

I recommend todetermine which is the best foryour needs from the datasheet and protocol information. Please take not of the different ranges these kits are suitable for. I suggest to choose the kits that are most suitable for the amounts of Fas ligand and CD95 you are expecting in your samples.

I believe that all these kits are suitable for un-bound (free) Fas ligand and CD95 since for ELISA the cells are lysed and ELISA is not a cell based assay.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank for your quick response. As I have noted in my previous e-mail, the kit seems to be working since the standard curve obtained is fine. The actual standard values are concentration-dependent and the curve is linear. We assayed over 50 healthy donors with ab45922 (CD95 Human ELISA Kit (1 x 96 Well Plate) and obtained the following concentration range: < 47pg/ml to 296pg/ml. We have not used it in any pathological samples, though. I hope this will be useful for you.  

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Answer

Thank for your enquiry regarding ab45922 and for providing some data saved in Excel file. After reviewing the results you have kindly sent to us, the standard curve obtained is fair and it seems the kit worked fine. Could you please confirm that you have used human samples and were expecting to have results within the range indicated in the precision paragraph of the insert? If so, this may not be appropriate. The data performance for precision is not an expected range for normal human serum or plasmas samples. This is only a reproducibility study made with spiked with recombinant protein serum. I hope this helps and if I can assist further, please do not hesitate to contact me.       

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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