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Human CD97 ELISA Kit (ab213763)

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Human CD97 ELISA Kit (ab213763) Standard Curve.

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 125 pg/ml - 8000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Recombinant Human CD97 protein (ab131865)

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    Overview

    • Product name

      Human CD97 ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      1 16 192pg/ml 12.09 = 7%
      2 16 1210pg/ml 62.92 = 5.2%
      3 16 5765ng/ml 253.66 = 4.4%
      Inter-assay
      Sample n Mean SD CV%
      1 24 193pg/ml 13.12 = 6.8%
      2 24 1108pg/ml 77.56 = 7%
      3 24 6274pg/ml 288.6 = 4.6%
    • Sample type

      Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 10 pg/ml
    • Range

      125 pg/ml - 8000 pg/ml
    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      The Human CD97 Enzyme-Linked Immunosorbent Assay (ELISA) Kit (ab213763) is designed for the quantitative measurement of Human CD97 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).


      The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD97 has been pre-coated onto 96-well plates. Standards (NSO, Q21-Q398) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD97 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD97 amount of sample captured in plate.

    • Notes

      Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Human CD97 coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
      Biotinylated anti- Human CD97 antibody 1 x 130µl
      Lyophilized recombinant Human CD97 standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Receptors / Channels
      • GPCR
      • More GPCR
      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR
      • Stem Cells
      • Hematopoietic Progenitors
      • Intracellular Molecules
    • Function

      Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
    • Tissue specificity

      Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
      Contains 5 EGF-like domains.
      Contains 1 GPS domain.
    • Domain

      The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
      Binding to chondroitin sulfate is mediated by the fourth EGF domain.
    • Post-translational
      modifications

      Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.
    • Cellular localization

      Cell membrane and Secreted > extracellular space.
    • Target information above from: UniProt accession P48960 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • ADGRE5
      • Adhesion G protein-coupled receptor E5
      • CD 97
      • CD_antigen=CD97
      • CD97
      • CD97 antigen
      • CD97 antigen subunit beta
      • CD97 molecule
      • CD97_HUMAN
      • Leukocyte antigen CD97
      • Seven span transmembrane protein
      • Seven transmembrane helix receptor
      • Seven transmembrane heterodimeric receptor associated with inflammation
      • TM7LN 1
      • TM7LN1
      see all
    • Database links

      • Entrez Gene: 976 Human
      • Omim: 601211 Human
      • SwissProt: P48960 Human
      • Unigene: 466039 Human

      Associated products

        Images

        • Human CD97 ELISA Kit (ab213763) Standard Curve.
          Human CD97 ELISA Kit (ab213763) Standard Curve.

          Human CD97 ELISA Kit (ab213763) Standard Curve.

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (0)

        Publishing research using ab213763? Please let us know so that we can cite the reference in this datasheet.

        ab213763 has not yet been referenced specifically in any publications.

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