Human CD97 ELISA Kit (ab213763)
- Datasheet
- References
- Protocols
Overview
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Product name
Human CD97 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 199pg/ml 11.54 5.8% 2 16 894pg/ml 52.74 5.9% 3 16 2165pg/ml 134.23 6.2% Inter-assay Sample n Mean SD CV% 1 24 201pg/ml 12.86 6.4% 2 24 715pg/ml 47.19 6.6% 3 24 3013pg/ml 213.92 7.1% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human CD97 Enzyme-Linked Immunosorbent Assay (ELISA) Kit (ab213763) is designed for the quantitative measurement of Human CD97 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CD97 has been pre-coated onto 96-well plates. Standards (NSO, Q21-Q398) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD97 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CD97 amount of sample captured in plate.
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Notes
Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human CD97 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti- Human CD97 antibody 1 x 130µl Lyophilized recombinant Human CD97 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. -
Tissue specificity
Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
Contains 5 EGF-like domains.
Contains 1 GPS domain. -
Domain
The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
Binding to chondroitin sulfate is mediated by the fourth EGF domain. -
Post-translational
modificationsProteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Alternative names
- ADGRE5
- Adhesion G protein-coupled receptor E5
- CD 97
see all -
Database links
- Entrez Gene: 976 Human
- Omim: 601211 Human
- SwissProt: P48960 Human
- Unigene: 466039 Human
Applications
Our Abpromise guarantee covers the use of ab213763 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Human CD97 ELISA Kit (ab213763) Standard Curve.Human CD97 ELISA Kit (ab213763) Standard Curve.
Protocols
References
ab213763 has not yet been referenced specifically in any publications.
