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    human-cdc25c-knockout-hela-cell-lysate-ab257387.pdf

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Cell Biology Cell Cycle Kinases/Phosphatases Phosphatases
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Human CDC25C knockout HeLa cell lysate (ab257387)

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Western blot - Human CDC25C knockout HeLa cell lysate (ab257387)
  • Western blot - Human CDC25C knockout HeLa cell lysate (ab257387)
  • Sanger Sequencing - Human CDC25C knockout HeLa cell lysate (ab257387)
  • Sanger Sequencing - Human CDC25C knockout HeLa cell lysate (ab257387)

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Overview

  • Product name

    Human CDC25C knockout HeLa cell lysate
    See all Cdc25C kits
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HeLa
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 2 bp insertion in exon2.
  • Passage number

    <20
  • Knockout validation

    Sanger Sequencing, Western Blot (WB)
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab262834 - Human CDC25C knockout HeLa cell lysate 1 x 100µg
    ab255929 - Human wild-type HeLa cell lysate 1 x 100µg
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Phospatases
  • Cell type

    epithelial
  • Disease

    Adenocarcinoma
  • Gender

    Female
  • STR Analysis

    Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10

Target

  • Function

    Functions as a dosage-dependent inducer in mitotic control. It is a tyrosine protein phosphatase required for progression of the cell cycle. It directly dephosphorylates CDK1 and activate its kinase activity.
  • Sequence similarities

    Belongs to the MPI phosphatase family.
    Contains 1 rhodanese domain.
  • Developmental stage

    Expressed predominantly in G2 phase.
  • Post-translational
    modifications

    Phosphorylated by CHK1 on Ser-216. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase. Phosphorylated by PLK4.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P30307 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • CDC 25
    • Cdc 25C
    • CDC25
    • CDC25C
    • Cell division cycle 25 homolog C
    • Cell division cycle 25C
    • Cell division cycle 25C protein
    • Dual specificity phosphatase Cdc25C
    • M phase inducer phosphatase 3
    • M-phase inducer phosphatase 3
    • Mitosis inducer CDC25
    • MPIP3
    • MPIP3_HUMAN
    • Phosphotyrosine phosphatase
    • PPP1R60
    • protein phosphatase 1, regulatory subunit 60
    see all

Associated products

  • KO cell lines

    • Human CDC25C knockout HeLa cell line (ab265189)
  • Related Products

    • Anti-Cdc25C antibody [E302] - BSA and Azide free (ab232553)
    • Anti-Cdc25C antibody [E303] - BSA and Azide free (ab247215)
    • Anti-Cdc25C antibody [E303] (ab32050)
    • Anti-Cdc25C antibody [E302] (ab32444)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab257387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.

Images

  • Western blot - Human CDC25C knockout HeLa cell lysate (ab257387)
    Western blot - Human CDC25C knockout HeLa cell lysate (ab257387)
    Lane 1: Wild-type HeLa cell lysate (20µg)

    Lane 2: CDC25C knockout HeLa cell lysate (20µg)

    Lanes 1- 2: Merged signal (red and green). Green - ab32444 observed at 58 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab32444 Anti-Cdc25C antibody [E302] was shown to specifically react with Cdc25C in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265189 (knockout cell lysate ab257387) was used. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32444 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Western blot - Human CDC25C knockout HeLa cell lysate (ab257387)
    Western blot - Human CDC25C knockout HeLa cell lysate (ab257387)
    Lane 1: Wild-type HeLa cell lysate (20µg)

    Lane 2: CDC25C knockout HeLa cell lysate (20µg)

    Lanes 1- 2: Merged signal (red and green). Green - ab32050 observed at 58 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab32050 Anti-Cdc25C antibody [E303] was shown to specifically react with Cdc25C in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265189 (knockout cell lysate ab257387) was used. Wild-type and Cdc25C knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32050 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
  • Sanger Sequencing - Human CDC25C knockout HeLa cell lysate (ab257387)
    Sanger Sequencing - Human CDC25C knockout HeLa cell lysate (ab257387)

    Allele-1: 1 bp insertion in exon2

     

  • Sanger Sequencing - Human CDC25C knockout HeLa cell lysate (ab257387)
    Sanger Sequencing - Human CDC25C knockout HeLa cell lysate (ab257387)

    Allele-2: 2 bp insertion in exon2

     

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab257387? Please let us know so that we can cite the reference in this datasheet.

ab257387 has not yet been referenced specifically in any publications.

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