Overview

  • Product name

    Human CDNF ELISA kit
    See all CDNF kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 15 677pg/ml 3.7%
    2 15 272pg/ml 2.8%
    3 15 167pg/ml 2.5%
    Inter-assay
    Sample n Mean SD CV%
    1 2 19pg/ml 8.1%
    2 2 155pg/ml 16%
    3 2 439pg/ml 20.8%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1 pg/ml
  • Range

    15 pg/ml - 960 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Spike 92.9 0pg/ml - 960pg/ml

  • Assay time

    2h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The human CDNF ELISA kit (ab215415) provides a rapid and easy method for the quantitative determination of human CDNF in cell culture supernatant, serum and plasma. The kit includes ready-to-use reagents necessary to analyze up to 88 samples in 2 hours.


     

  • Notes

    Microplate wells are pre-coated with anti-human CDNF-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human CDNF molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human CDNF-specific monoclonal detection antibodies bind to a different epitope of human CDNF molecules. After washing, the ready to use HRP substrate (TMB) is added to wells. The intensity of the color produced is directly proportional to the amount of human CDNF in the sample. Color development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.

     

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    anti-Human CDNF coated microplate (12 x 8 wells) 1 x 96 tests
    10X Wash concentrate 1 x 50ml
    Human CDNF enzyme conjugate 1 x 12ml
    Human CDNF sample diluent 1 x 25ml
    Human CDNF standard A (0 pg/mL) 1 x 1ml
    Human CDNF standard B (15 pg/mL) 1 x 1ml
    Human CDNF standard C (30 pg/mL) 1 x 1ml
    Human CDNF standard D (60 pg/mL) 1 x 1ml
    Human CDNF standard E (120 pg/mL) 1 x 1ml
    Human CDNF standard F (240 pg/mL) 1 x 1ml
    Human CDNF standard G (480 pg/mL) 1 x 1ml
    Human CDNF standard H (960 pg/mL) 1 x 1ml
    Stop solution 1 x 12ml
    TMB Substrate solution 1 x 12ml
  • Research areas

  • Function

    Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons in substantia nigra.
  • Tissue specificity

    Widely expressed in neuronal and non-neuronal tissues. In the brain, highest levels in the optic nerve and corpus callosum.
  • Sequence similarities

    Belongs to the ARMET family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Arginine rich mutated in early stage tumors like 1
    • ARMET L1
    • ARMET like protein 1
    • ARMET-like protein 1
    • ARMETL 1
    • ARMETL1
    • CDNF
    • CDNF_HUMAN
    • Cerebral dopamine neurotrophic factor
    • Conserved dopamine neurotrophic factor
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab215415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human CDNF ELISA kit ab215415. Linear regression (LLS) fit curve on a lin-lin scale

  • Human CDNF ELISA kit ab215415. Standard Curve, Cubic spline fit curve on a log-log scale.

Protocols

References

ab215415 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215415.
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