Overview

  • Product name

    Human CEACAM1 ELISA Kit
    See all CEACAM1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.7%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    9.1 pg/ml
  • Range

    46.9 pg/ml - 3000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 102 98% - 106%
    Cell culture extracts 100 97% - 102%
    Cell culture media 110 107% - 113%
    Heparin Plasma 101 95% - 106%
    EDTA Plasma 101 93% - 109%
    Citrate Plasma 96 89% - 100%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    CEACAM1 in vitro SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CEACAM1 protein in serum, plasma, cell culture supernatant, cell and tissue extracts.


    The SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in Sample Diluent NS: 9.1 pg/mL
    Samples in 1X Cell Extraction Buffer PTR: 10.3 pg/mL

  • Notes

    CEACAM1 (Carcinoembryonic antigen-related cell adhesion molecule 1 or BGP-1 or CD66a) is a member of the CEA family of cell surface transmembrane proteins. The antibodies in this kit were raised to the full extracellular domain of CEACAM1.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human CEACAM1 Capture Antibody 1 x 600µl
    10X Human CEACAM1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Human CEACAM1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. CEA family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact.
  • Information by UniProt
  • Alternative names

    • Antigen CD66
    • BGP
    • BGP 1
    • BGP-1
    • BGPI
    • Biliary glycoprotein
    • Biliary glycoprotein 1
    • Biliary glycoprotein adhesion molecule
    • Carcinoembryonic antigen related cell adhesion molecule 1
    • Carcinoembryonic antigen-related cell adhesion molecule 1
    • carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
    • CD66a
    • CD66a antigen
    • CEACAM1
    • CEAM1_HUMAN
    • meconium antigen 100
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab215540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CEACAM1 were measured in duplicates, interpolated from the CEACAM1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEACAM1 concentration was determined to be 2,109 pg/mL in serum, 1,783 pg/mL in plasma (citrate), 1,729 pg/mL in plasma (EDTA) and 1,818 pg/mL in plasma (heparin).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEACAM1 concentration was determined to be 1940 pg/mL with a range of 1413 – 2640 pg/mL.

  • The concentrations of CEACAM1 were measured in duplicates, interpolated from the CEACAM1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEACAM1 concentration was determined to be 2,109 pg/mL in cell culture supernatant.

  • The concentrations of CEACAM1 were measured in duplicate and interpolated from the CEACAM1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEACAM1 concentration was determined to be 1,022 pg/mL in HEPG2 cell extract.

Protocols

References

ab215540 has not yet been referenced specifically in any publications.

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