Product nameHuman CEACAM3 knockout A549 cell lysate
Cell line information
Parental cell line: A549
Organism: Homo sapiens
Gene editing information
Editing tool: CRISPR/Cas9
Mutation: 109 bp deletion in exon1 and 1 bp insertion in exon1 and 23 bp deletion in exon1.
Knockout validation: Confirmed by Sanger sequencing.
Reconstitution instructions: To use as a WB control, resuspend in 45 µL of Sample buffer (40% (w/v) Glycerol, 4% (w/v) Lithium Dodecyl Sulfate, 4% Ficoll 400, 0.025% Phenol Red, 0.025% Brilliant Blue G250, 2 mM EDTA) and 5 µL of DTT to resuspend @ 2mg/ml. Mix well, then boil the sample for 10 minutes before loading it onto the gel.
User storage instructions: Upon receiving, lysate can be diluted with 1 x SDS sample buffer & will be stable at -20°C for 12 months. Long term storage at -80°C.
Knockout validationSanger Sequencing
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Storage instructionsStore at -80°C. Please refer to protocols.
Components 1 kit Human CEACAM3 knockout A549 cell lysate (Lyophilized) 1 x 100µg Human Wild Type A549 cell lysate (Lyophilized) 1 x 100µg
FunctionMajor granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis.
Tissue specificityCGM1a, the predominant CGM1 transcript, is granulocyte-specific. Not detected out of the granulocytic lineage, such as monocytes, lymphocytes, spleen, testis, colon, brain, liver, pancreas, thymus, ovary, placenta, skeletal muscle, prostate, small intestine, heart, lung and kidney.
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
DomainThe cytosolic domain is involved in S100A9 interaction.
modificationsTyrosine-phosphorylated in response to microbial binding. Tyr-230 and Tyr-241 are both required for phosphorylation to be detected.
- Information by UniProt
- Carcinoembryonic antigen CGM1
- Carcinoembryonic antigen gene family member 1
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263146 has not yet been referenced specifically in any publications.