Overview

  • Product name

    Human Ceruloplasmin ELISA Kit
    See all Ceruloplasmin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human serum 7 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 4.7%
  • Sample type

    Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    80 pg/ml
  • Range

    230 pg/ml - 15000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Milk 95 81% - 106%
    Urine 98 91% - 103%
    Serum 92 80% - 110%
    Hep Plasma 103 96% - 111%
    EDTA Plasma 91 76% - 102%
    Cit plasma 98 85% - 113%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Ceruloplasmin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Ceruloplasmin protein in human serum, plasma, milk, and urine.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Ceruloplasmin, also known as ferroxidase, is a 1046- amino acid glycosylated enzyme encoded by the gene CP. Ceruloplasmin is synthesized by the liver and secreted into the plasma, where it is the primary copper-carrying protein in blood. A monomer of the protein is capable of chelating 6 copper ions. Ceruloplasmin is capable of oxidizing Fe2+ to Fe3+ without generating radical oxygen species, which assists with the transport of iron across the cellular membrane. Low ceruloplasmin levels in plasma are associated with hepatic disease, Wilson disease, Menkes disease, copper deficiency, and aceruloplasminemia.  

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Ceruloplasmin Capture Antibody 1 x 600µl
    10X Human Ceruloplasmin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    Antibody Diluent CPR 1 x 6ml
    Denaturant 2 x 500µl
    Human Ceruloplasmin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Involvement in disease

    Defects in CP are the cause of aceruloplasminemia (ACERULOP) [MIM:604290]. It is an autosomal recessive disorder of iron metabolism characterized by iron accumulation in the brain as well as visceral organs. Clinical features consist of the triad of retinal degeneration, diabetes mellitus and neurological disturbances.
    Note=Ceruloplasmin levels are decreased in Wilson disease, in which copper cannot be incorporated into ceruloplasmin in liver because of defects in the copper-transporting ATPase 2.
  • Sequence similarities

    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 6 plastocyanin-like domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • CERU_HUMAN
    • Ceruloplasmin
    • CP
    • CP 2
    • CP2
    • Ferroxidase
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab236718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The Ceruloplasmin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Ceruloplasmin were measured in duplicates, interpolated from the Ceruloplasmin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 0.0031%, plasma (EDTA) 0.0063%, plasma (citrate) 0.0063%, and plasma (heparin) 0.0031%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ceruloplasmin concentration was determined to be 365 µg/mL in serum, 200 µg/mL in plasma (EDTA), 247 µg/mL in plasma (citrate), and 298 µg/mL in plasma (Heparin).

  • The concentrations of Ceruloplasmin were measured in duplicates, interpolated from the Ceruloplasmin standard curves and corrected for sample dilution. Undiluted samples are as follows: milk 1% and urine 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ceruloplasmin concentration was determined to be 1,888 ng/mL in milk and 162 ng/mL in urine.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ceruloplasmin concentration was determined to be 462 µg/mL with a range of 261– 683 µg/mL.

Protocols

References

ab236718 has not yet been referenced specifically in any publications.

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