Human Chemokine Antibody Array (40 Targets) - Quantitative (ab197435)
Overview
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Product name
Human Chemokine Antibody Array (40 Targets) - Quantitative
See all Chemokine Antibody Array antibody arrays -
Sample type
Serum, Plasma, Other biological fluids, Cell Lysate, Cell culture media, Tissue Lysate -
Assay type
Quantitative -
Assay time
6h 00m -
Species reactivity
Reacts with: Human -
Product overview
ab197435 can be used for quantitative measurement of 40 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates.
Target names: 6Ckine, Axl, BTC, CCL28, CTACK, CXCL16, ENA-78, Eotaxin-3, GCP-2, GRO, HCC-1, HCC-4, IL-9, IL-17F, IL-18 BPa, IL-28A, IL-29, IL-31, IP-10, I-TAC, LIF, LIGHT, Lymphotactin, MCP-2, MCP-3, MCP-4, MDC, MIF, MIP-3α, MIP-3β, MPIF-1, MSPα, NAP-2, OPN, PARC, PF4, SDF-1α, TARC, TECK, TSLP.
The analytes measured by this kit are split across a single array on the same slide, provided in the kit.
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Notes
Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins.
Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array.Each antibody is spotted in quadruplicate on each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 36 with 3 slides etc.
For high through-put, 4 slides can be nested into a tray matching a standard microplate (not supplied), allowing automated processing with a liquid handling workstation.
Array processing can be completed within one working day and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners (see scanner requirements at www.abcam.com/QuantAntibodyArrays). If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader. See below for a schematic protocol, the fluorophore is a dye with equivalent to excitation/ emission to Cy3.
The standard is affinity purified.
Learn more about quantitative antibody arrays
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 8 tests 22 tests 50 tests 20X Wash Buffer I 2 x 30ml 3 x 30ml 6 x 30ml 20X Wash Buffer II 1 x 30ml 1 x 30ml 2 x 30ml Cy3 equivalent dye-conjugated Streptavidin 1 x 5µl 2 x 5µl 4 x 5µl Human Chemokine Array (40 T) Biotinylated Antibody Cocktail 1 unit 2 units 4 units Human Chemokine Array (40 T) Glass Slide 1 slide 2 slides 4 slides Human Chemokine Array (40 T) Lyophilized Cytokine Standard Mix 1 vial 1 vial 2 vials Sample Diluent 1 x 15ml 1 x 15ml 2 x 15ml Slide Washer/Dryer (30 mL Centrifuge Tube) 1 unit 1 unit 2 units
Images
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Assay summary for Abcam Human Chemokine Antibody Array (40 Targets) - Quantitative (ab197435).
Assay summary for Abcam Human Chemokine Antibody Array (40 Targets) - Quantitative (ab197435).
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Array map for Abcam Human Chemokine Antibody Array (40 Targets) - Quantitative (ab197435).Array map for Abcam Human Chemokine Antibody Array (40 Targets) - Quantitative (ab197435).
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Standard Curves obtained using Abcam Human Chemokine Antibody Array (40 Targets) - Quantitative (ab197435).Standard Curves obtained using Abcam Human Chemokine Antibody Array (40 Targets) - Quantitative (ab197435).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab197435 has not yet been referenced specifically in any publications.