Overview

  • Product name
    Human Anti-Chikungunya Virus IgG ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Low + serum 23 9.4%
    High + serum 24 2.7%
    Inter-assay
    Sample n Mean SD CV%
    Low + serum 3 10.9%
    High + serum 3 3.7%
  • Sample type
    Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Sandwich (qualitative)
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Chikungunya Virus IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Chikungunya Virus in Human serum and plasma.

    A 96-well plate has been precoated with anti-Human antibodies to bind to corresponding antibodies of the sample. Controls or test samples are added to the wells and incubated. Following washing, the Chikungunya Virus antigen is added to the wells and incubated. The wells are then washed as before and the biotinylated Chikungunya Virus antibodies are added to each well followed by incubation. After a last wash step, the streptavidin peroxidase (SP) conjugate which binds to the biotinylated Chikungunya Virus-specific antibodies is added to the wells. TMB is then catalyzed by the SP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chikungunya Virus IgG sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab177835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:
  • Weber C  et al. A small antigenic determinant of the Chikungunya virus E2 protein is sufficient to induce neutralizing antibodies which are partially protective in mice. PLoS Negl Trop Dis 9:e0003684 (2015). WB ; Human . Read more (PubMed: 25905779) »
  • Erasmus JH  et al. Utilization of an Eilat Virus-Based Chimera for Serological Detection of Chikungunya Infection. PLoS Negl Trop Dis 9:e0004119 (2015). Read more (PubMed: 26492074) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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