Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Chlamydia pneumoniae IgA ELISA Kit
Intra-assay Sample n Mean SD CV% Pos. Serum 20 4.9% Pos. Serum 24 8.3% Inter-assay Sample n Mean SD CV% Pos. Serum 12 9.7% Pos. Serum 12 14%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Chlamydia pneumoniae IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Chlamydia pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Chlamydia pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Chlamydia pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chlamydia pneumoniae IgA sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Chlamydia pneumoniae (IgA) Coated Microplate (12 x 8 wells) 1 unit Chlamydia pneumoniae anti-IgA HRP Conjugate Colored violet, black cap 1 x 20ml Chlamydia pneumoniae IgA Cut Off Control Colored yellow, green cap 1 x 3ml Chlamydia pneumoniae IgA Negative Control Colored yellow, blue cap 1 x 2ml Chlamydia pneumoniae IgA Positive Control Colored yellow, red cap. 1 x 2ml IgA Sample Diluent Colored yellow; white cap 1 x 100ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml
ab108719 has not yet been referenced specifically in any publications.