Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Chlamydia pneumoniae IgM ELISA Kit
Intra-assay Sample n Mean SD CV% Pos. Serum 24 4.01% Inter-assay Sample n Mean SD CV% Pos. Serum 12 8.25%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Chlamydia pneumoniae IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Chlamydia pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Chlamydia pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Chlamydia pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chlamydia pneumoniae IgM sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Chlamydia pneumoniae (IgM) Coated Microplate (12 x 8 wells) 1 unit Chlamydia pneumoniae anti-IgM HRP Conjugate Black cap 1 x 20ml Chlamydia pneumoniae IgM Cut-off Control Green cap 1 x 3ml Chlamydia pneumoniae IgM Negative Control Blue cap 1 x 2ml Chlamydia pneumoniae IgM Positive Control Red cap 1 x 2ml Cover foil 1 unit IgM Sample Diluent White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab108723 has not yet been referenced specifically in any publications.