Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Chlamydia trachomatis IgA ELISA Kit
Intra-assay Sample n Mean SD CV% Pos. Serum 24 4.7% Inter-assay Sample n Mean SD CV% Pos. Serum 12 7.1%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Chlamydia trachomatis IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Chlamydia trachomatis in Human serum and plasma.
A 96-well plate has been precoated with Chlamydia trachomatis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Chlamydia trachomatis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chlamydia trachomatis IgA sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Chlamydia trachomatis (IgA) Coated Microplate (12 x 8 wells) 1 unit Chlamydia trachomatis anti-IgA HRP Conjugate Violet Bottle, Black Cap 1 x 20ml Chlamydia trachomatis IgA Cut-off Control Yellow Bottle, Green Cap 1 x 3ml Chlamydia trachomatis IgA Negative Control Yellow Bottle, Blue Cap 1 x 2ml Chlamydia trachomatis IgA Positive Control Yellow bottle, Red Cap 1 x 2ml Cover foil 1 unit IgA Sample Diluent White Cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
ab108718 has not yet been referenced specifically in any publications.