Overview

  • Product name

    Human Anti-Chlamydia trachomatis IgG ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Pos. Serum 24 3.7%
    Inter-assay
    Sample n Mean SD CV%
    Pos. Serum 12 2.8%
  • Sample type

    Serum, Heparin Plasma, Citrate Plasma
  • Assay type

    Indirect
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s anti-Chlamydia trachomatis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Chlamydia trachomatis in Human serum and plasma.

     

    A 96-well plate has been precoated with Chlamydia trachomatis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Chlamydia trachomatis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chlamydia trachomatis IgG sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:

  • Xin H  et al. Diagnostic value of detection of serum ß-HCG and CT-IgG combined with transvaginal ultrasonography in early tubal pregnancy. Exp Ther Med 16:277-281 (2018). Read more (PubMed: 29896250) »
See 1 Publication for this product

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