Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Chlamydia trachomatis IgG ELISA Kit
Intra-assay Sample n Mean SD CV% Pos. Serum 24 3.7% Inter-assay Sample n Mean SD CV% Pos. Serum 12 2.8%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Chlamydia trachomatis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Chlamydia trachomatis in Human serum and plasma.
A 96-well plate has been precoated with Chlamydia trachomatis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Chlamydia trachomatis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Chlamydia trachomatis IgG sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Chlamydia trachomatis (IgG) Coated Microplate (12 x 8 wells) 1 unit Chlamydia trachomatis anti-IgG HRP Conjugate Black cap 1 x 20ml Chlamydia trachomatis IgG Cut-off Control Green cap 1 x 3ml Chlamydia trachomatis IgG Negative Control Blue cap 1 x 2ml Chlamydia trachomatis IgG Positive Control Red cap 1 x 2ml Cover foil 1 unit IgG Sample Diluent White cap 1 x 100ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
ab108720 has been referenced in 1 publication.
- Xin H et al. Diagnostic value of detection of serum ß-HCG and CT-IgG combined with transvaginal ultrasonography in early tubal pregnancy. Exp Ther Med 16:277-281 (2018). PubMed: 29896250