Key features and details
- Sensitivity: 0.09 mU/ml
- Range: 1 mU/ml - 400 mU/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Chorionic Gonadotropin beta ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 48 < 7.6% Inter-assay Sample n Mean SD CV% Overall 3 < 8.8%
Sample typeSerum, Hep Plasma, Cit plasma
Assay typeSandwich (quantitative)
Sensitivity> 0.09 mU/ml
Range1 mU/ml - 400 mU/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Chorionic Gonadotropin beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Chorionic Gonadotropin beta in Human serum and plasma.
A 96-well plate has been precoated with anti-Chorionic Gonadotropin beta IgG antibodies. Samples, standards and the Chorionic Gonadotropin beta-HRP conjugate are added to the wells, where Chorionic Gonadotropin beta in the sample and standards binds to the precoated antibody and added Chorionic Gonadotropin beta-HRP conjugate binds to this antibody-Chorionic Gonadotropin beta complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Chorionic Gonadotropin beta in the sample and the intensity is measured at 450 nm.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Anti-Chorionic Gonadotropin beta HRP Conjugate 1 x 1ml 50X Washing Solution 1 x 20ml Anti-Chorionic Gonadotropin beta Coated Microplate (12 x 8 wells) 1 unit Chorionic Gonadotrophin beta Control 1 x 1ml Chorionic Gonadotropin beta Standard 0 - 0 mU/mL 1 x 1ml Chorionic Gonadotropin beta Standard 1 - 1 mU/mL 1 x 1ml Chorionic Gonadotropin beta Standard 2 - 5 mU/mL 1 x 1ml Chorionic Gonadotropin beta Standard 3 - 20 mU/mL 1 x 1ml Chorionic Gonadotropin beta Standard 4 - 100 mU/mL 1 x 1ml Chorionic Gonadotropin beta Standard 5 - 400 mU/mL 1 x 1ml Cover Foil 1 unit Incubation Buffer 1 x 50ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml
- CG beta
- SwissProt: P01233 Human
ab178633 has not yet been referenced specifically in any publications.