Overview

  • Product name

    Human Chromogranin A ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    EDTA Plasma 8 5.87%
    Inter-assay
    Sample n Mean SD CV%
    EDTA Plasma 3 5.03%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    168 pg/ml
  • Range

    0.195 ng/ml - 12.5 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 110 105% - 116%
    Cell culture media 93 85% - 99%
    Heparin Plasma 113 106% - 119%
    EDTA Plasma 114 110% - 118%
    Citrate Plasma 117 113% - 122%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Chromogranin A in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human serum, plasma, and cell culture supernatant samples.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Chromogranin A (CHGA, CgA, also known as Parathyroid secretory protein 1) is a member of the granin family, which are acidic proteins found in the secretory granules of endocrine cells. CHGA consists of ~460 amino acids, and is processed into numerous peptides that have diverse activities. Some of the peptides affect the secretion of other hormones, regulate metabolism, and regulate neural pathways that control behavior. Research has shown CHGA to be elevated in the blood of cancer patients, to inhibit angiogenesis, and affect tumor response to chemotherapy. It is a popular biomarker for Neuroendocrine tumors, and increased levels have been detected in patients with lung cancer, prostate cancer, and breast cancer. Elevated CHGA serum levels have also been detected in non-cancer disease state patients with heart and renal failure, hypertension, rheumatoid arthritis, and various other inflammatory diseases.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Chromogranin A Capture Antibody 1 x 600µl
    10X Human Chromogranin A Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human Chromogranin A Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Function

    Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • Sequence similarities

    Belongs to the chromogranin/secretogranin protein family.
  • Post-translational
    modifications

    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle membrane. Secreted. Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions.
  • Information by UniProt
  • Alternative names

    • Catestatin
    • CgA
    • Chga
    • CMGA_HUMAN
    • ER-37
    • Parathyroid secretory protein 1
    • Pituitary secretory protein I
    • SP-I
    • Vasostatin I
    • Vasostatin II
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab196271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Non-background subtracted data from duplicate measurements are plotted. Chromogranin A was detectable in SY5Y cell culture supernatants but not HeLa cell culture.

  • Duplicate interpolated values of Chromogranin A are graphed. Medical histories of individual donors were not known. Mean Chromogranin A concentration observed was 10.57 ng/mL.

Protocols

References

This product has been referenced in:

  • Hofving T  et al. 177Lu-octreotate therapy for neuroendocrine tumours is enhanced by Hsp90 inhibition. Endocr Relat Cancer N/A:N/A (2019). Read more (PubMed: 30730850) »
  • Curtin C  et al. A physiologically relevant 3D collagen-based scaffold-neuroblastoma cell system exhibits chemosensitivity similar to orthotopic xenograft models. Acta Biomater 70:84-97 (2018). Read more (PubMed: 29447961) »
See all 3 Publications for this product

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