Human CKM ELISA Kit (ab185988)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Human CKM ELISA Kit
See all Creatine Kinase MM kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 4 3% Inter-assay Sample n Mean SD CV% Human serum 3 9% -
Sample type
Urine, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Recovery
Sample specific recovery Sample type Average % Range Urine 98 87% - 114% Serum 89 82% - 99% Plasma 115 93% - 140% Extraction Buffer 91 72% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
CKM in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CKM protein in human serum, plasma, urine, tissue and cell extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Samples diluted in Sample Diluent NS - 0.014 U/L
Samples diluted in 1X Cell Extraction Buffer PTR - 0.007 U/L
Range:
0.03 U/L - 2U/L -
Notes
Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase, is an enzyme that catalyzes the reversible transfer of a high-energy phosphate group between phosphocreatine and ATP. There are five known CK isozymes, three located in the cytosol (CKMM, CKMB, CKBB) and two in the mitochondria (MiSCK and MiuCK). Structurally, cytosolic CK is a 86kDa dimeric enzyme of two identical or non-identical chains, CKM and/or CKB whereas mitochondrial CK is an octameric enzyme associated with mitochondrial membranes. The compartmentation of CK isoenzymes allows for direct association with ATP-providing or consuming processes both linked via metabolite channeling through the creatine phosphate shuttle. Thus CK isoenzymes allow for the generation of a large pool of phosphocreatine, considered to be a temporal and spatial ATP buffering system in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 10X CKM Capture Antibody 1 x 600µl 10X CKM Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human CKM Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Cytoplasmic -
Alternative names
- CKM
- CKMM
- creatine kinase M chain
see all -
Database links
- Entrez Gene: 1158 Human
- Omim: 123310 Human
- SwissProt: P06732 Human
- Unigene: 334347 Human
Applications
Our Abpromise guarantee covers the use of ab185988 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Human CKM ELISA Kit (ab185988)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of CKM standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of CKM standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Ten individual healthy donors were evaluated for the presence of CKM in serum using this assay.Results were interpolated from the standard curve in Sample Diluent NS and corrected by sample dilution (50 fold). The mean level of CKM is 115.3 U/L with a range of 54.3 – 268.9 U/L and a standard deviation of 77.8U/L.
Protocols
References
ab185988 has not yet been referenced specifically in any publications.
