Overview

  • Product name

    Human CKMB ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Serum, Plasma, Cell culture media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.3 ng/ml
  • Range

    0.3 ng/ml - 80 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 97.17 71% - 123%
    Plasma 87.57 78% - 108%
    Cell culture media 86.45 67% - 106%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam's CKMB Human ELISA Kit (ab193696) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CKMB in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human CKMB coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CKMB present in the samples. The wells are washed and biotinylated anti-Human CKMB antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CKMB bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent D 1 x 15ml
    5X Assay Diluent B 1 x 15ml
    Biotinylated Human CKMB detection antibody (lyophilized) 2 vials
    Human CKMB Standards (lyophilized) 2 vials
    Pre-coated Human CKMB Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities

    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Alternative names

    • CKM
    • Creatine kinase M chain
    • Creatine kinase M-type
    • KCRM_HUMAN
    • M-CK
    • N-terminally processed
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:

  • Zhang Z  et al. Astragalus mongholicus (Fisch.) Bge Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155. Front Pediatr 6:139 (2018). Read more (PubMed: 29977885) »
See 1 Publication for this product

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