Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.3 ng/ml - 80 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman CKMB ELISA Kit
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeSerum, Plasma, Cell culture media
Assay typeSandwich (quantitative)
Range0.3 ng/ml - 80 ng/ml
Sample specific recovery Sample type Average % Range Serum 97.17 71% - 123% Plasma 87.57 78% - 108% Cell culture media 86.45 67% - 106%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam's CKMB Human ELISA Kit (ab193696) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CKMB in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CKMB coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CKMB present in the samples. The wells are washed and biotinylated anti-Human CKMB antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CKMB bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent D 1 x 15ml 5X Assay Diluent B 1 x 15ml Biotinylated Human CKMB detection antibody (lyophilized) 2 vials Human CKMB Standards (lyophilized) 2 vials Pre-coated Human CKMB Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain.
- Information by UniProt
- Creatine kinase M chain
- Creatine kinase M-type
ab193696 has been referenced in 1 publication.
- Zhang Z et al. Astragalus mongholicus (Fisch.) Bge Improves Peripheral Treg Cell Immunity Imbalance in the Children With Viral Myocarditis by Reducing the Levels of miR-146b and miR-155. Front Pediatr 6:139 (2018). PubMed: 29977885