Overview

  • Product name

    Human Anti-Clostridium tetani toxin IgG ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Weak + Serum 24 0.56U/ml 7.1%
    Pos. Serum 24 1.24U/ml 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Weak + Serum 10 0.22U/ml 11.3%
    Pos. Serum 10 0.54U/ml 7.7%
  • Sample type

    Serum, Hep Plasma, Cit plasma
  • Assay type

    Indirect
  • Sensitivity

    < 0.05 U/ml
  • Range

    0.05 U/ml - 1 U/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s anti-Clostridium tetani toxin IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Clostridium tetani toxin in Human serum and plasma.


    A 96-well plate has been precoated with Clostridium tetani toxin antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Clostridium tetani toxin-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Clostridium tetani toxin IgG sample captured in plate.

  • Tested applications

    Suitable for: Indirect ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent dilution.

Images

  • Representative Standard Curve using ab108771

Protocols

References

ab108771 has not yet been referenced specifically in any publications.

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