• Product name
    Human CNTF ELISA Kit
    See all CNTF kits
  • Detection method
  • Sample type
    Cell culture supernatant, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.2 ng/ml
  • Range
    0.823 ng/ml - 200 ng/ml
  • Recovery

    > 92 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.45 84% - 104%
    Plasma 93.28 83% - 104%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
    Does not react with: Rat
  • Product overview

    Abcam’s CNTF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CNTF in plasma and cell culture supernatants. (Human CNTF concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect endogenous Human CNTF in normal serum with ab99994, only in serum spiked with Human CNTF.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab99994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab99994

  • Representative Standard Curve using ab99994



ab99994 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Thank you for your inquiry. For the kit ab99994, the capture antibody was collected from mouse ascities after the mouse was injected with the immunogen (so the host is mouse). The host for the detection antibody is goa...

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Thank you for your inquiry.  Unfortunately, we have not tested mouse samples with the ab99994 kit or ab100758 kit. The lab didn't think that the human kit ab99994 would detect mouse samples since it didn't work in rat (the mouse and rat proteins ar...

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I apologize for the delay in getting back to you in regards to this issue, and I appreciate your patience as I have been discussing this situation with my colleagues. I can understand your frustration and disappointment as the results have been unexpec...

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Thank you very much for the data. I am working with some of my colleagues to resolve this as quickly as possible, and I appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Thank you for your reply. I have found the order with the additional kits that you mentioned. In order to process this refund, I will need a little bit more technical information. Have you tried all of these kits, or did you test 1 from each set and th...

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Thank you for your reply, and I apologize that these kits did not work well for you. I did not find the attached summary of the test results, could you please send these again? We do fully guarantee these kits to work, so I have issued credit note *** ...

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I am very pleased to hear you would like to accept our offer and test ab100758 in mouse. This code will give you 1 free kit OR 1 free antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code i...

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Thank you for your email.  I have addressed your question below. ab100758 : No cross reactivity data is available testing mouse samples with this rat kit therefore the only way to know would be to test and see.  However, both the capture and detection ...

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Thank you for contacting Abcam regarding ab99994. I have received some useful information from the lab regarding the detection of CNTF is plasma samples. CNTF is a fairly low abundant protein in human serum and plasma and therefore may be difficult...

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Thank you for calling Abcam. Regarding the preparation of plasma for use with this kit, please see the following protocol: - Collect blood into EDTA or Sodium heparin tube - Spin 10 minutes at 3000 rpm - Aliquot into small tubes and store at –...

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