Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.823 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman CNTF ELISA Kit
See all CNTF kits
Sample typeCell culture supernatant, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 0.2 ng/ml
Range0.823 ng/ml - 200 ng/ml
> 92 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.45 84% - 104% Plasma 93.28 83% - 104%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Does not react with: Rat
Abcam’s CNTF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CNTF in plasma and cell culture supernatants. (Human CNTF concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human CNTF coated on a 96-well plate. Standards and samples are pipetted into the wells and CNTF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CNTF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human CNTF in normal serum with ab99994, only in serum spiked with Human CNTF.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CNTF 2 vials CNTF Microplate (12 strips x 8 wells) 1 unit Recombinant Human CNTF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
RelevanceCiliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. In addition to its neuronal actions, CNTF also acts on non neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells.
- Ciliary Neurotrophic Factor
ab99994 has not yet been referenced specifically in any publications.