Overview

  • Product name

    Human Complement C1 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.1 ng/ml
  • Range

    0.125 ng/ml - 8 ng/ml
  • Recovery

    98 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow, Dog, Pig, Monkey
  • Product overview

    The Human Complement C1 ELISA (Enzyme-Linked Immunosorbent Assay) Kit (an222865) is designed for detection of C1 in human plasma, serum, saliva, urine, milk, and cell culture samples.


    This assay employs a quantitative sandwich enzyme immunoassay technique that measures C1 in less than 4 hours. A polyclonal antibody specific for C1 has been pre-coated onto a 96-well microplate with removable strips. C1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for C1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Complement component C1 (C1) is a calcium-dependent serine protease complex with an approximate mass of 790 kDa and acts as the first component of the classical complement pathway. C1 is formed from the association of a recognition protein C1q and two catalytic subunits C1r and C1s respectively. The globular heads of the C1q bind to the Fc-fragment of IgM or IgG on the surface of a pathogen, resulting in the activation of C1r. The activated C1r is able to activate C1s which in turn activates C2 and C4, leading to the production of the C4b-C2a form of C3-convertase. 

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab222865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of Human Complement C1 standard curve in Diluent N. Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab222865 has not yet been referenced specifically in any publications.

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