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    human-complement-c1r-elisa-kit-ab170245.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Human Complement C1r ELISA Kit (ab170245)

  • Datasheet
  • SDS
  • Protocol Booklet
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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.1 ng/ml
    • Range: 0.125 ng/ml - 8 ng/ml
    • Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Urine
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Human

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    Protein
    Product image
    Recombinant Human C1r protein (ab152248)

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    Overview

    • Product name

      Human Complement C1r ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall 2.6%
      Inter-assay
      Sample n Mean SD CV%
      Overall 8.8%
    • Sample type

      Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      > 0.1 ng/ml
    • Range

      0.125 ng/ml - 8 ng/ml
    • Recovery

      96 %

    • Assay time

      4h 00m
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Complement C1r Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C1r concentrations in plasma, serum, saliva, urine, milk and cell culture supernatants.


      A Complement C1r specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C1r specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C1r captured in plate.


      The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 1 x 96 tests
      100X Biotinylated Human Complement C1r Antibody 1 x 60µl
      100X Streptavidin-Peroxidase Conjugate 1 x 80µl
      10X Diluent M Concentrate 1 x 30ml
      20X Wash Buffer Concentrate 2 x 30ml
      Chromogen Substrate 1 x 8ml
      Complement C1r Microplate (12 x 8 well strips) 1 unit
      Complement C1r Standard 1 vial
      Sealing Tapes 3 units
      Stop Solution 1 x 12ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Complement
      • Classical Pathway
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Complement ELISA kits
    • Function

      C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 2 CUB domains.
      Contains 1 EGF-like domain.
      Contains 1 peptidase S1 domain.
      Contains 2 Sushi (CCP/SCR) domains.
    • Post-translational
      modifications

      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    • Target information above from: UniProt accession P00736 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • C1 R
      • C1R
      • C1R_HUMAN
      • Complement C1r
      • Complement C1r subcomponent
      • Complement C1r subcomponent light chain
      • Complement C1r subcomponent precursor
      • Complement component 1 r subcomponent
      • Complement component 1 subcomponent r
      see all
    • Database links

      • Entrez Gene: 715 Human
      • Omim: 613785 Human
      • SwissProt: P00736 Human
      • Unigene: 524224 Human

      Associated products

        Images

        • Typical Standard Curve
          Typical Standard Curve

          Representative Standard Curve using ab170245

        Protocols

        • Protocol Booklet

        Click here to view the general protocols

        Datasheets and documents

        • Datasheet
        • SDS
      • References (0)

        Publishing research using ab170245? Please let us know so that we can cite the reference in this datasheet.

        ab170245 has not yet been referenced specifically in any publications.

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