Human C1r ELISA Kit (ab170245)

Precision

Intra-assay
Sample	n	Mean	SD	CV%
Overall				2.6%

Inter-assay
Sample	n	Mean	SD	CV%
Overall				8.8%

Sample type

Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
Assay type

Sandwich (quantitative)
Sensitivity

> 0.1 ng/ml
Range

0.125 ng/ml - 8 ng/ml
Recovery

96 %
Assay time

4h 00m
Species reactivity

Reacts with: Human
Product overview

Abcam’s Complement C1r Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C1r concentrations in plasma, serum, saliva, urine, milk and cell culture supernatants.
A Complement C1r specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C1r specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C1r captured in plate.
Tested applications

Suitable for: Sandwich ELISAmore details

Components	1 x 96 tests
100X Streptavidin-Peroxidase Conjugate	1 x 80µl
10X Diluent M Concentrate	1 x 30ml
20X Wash Buffer Concentrate	2 x 30ml
50X Biotinylated Human Complement C1r Antibody	1 x 120µl
Chromogen Substrate	1 x 8ml
Complement C1r Microplate (12 x 8 well strips)	1 unit
Complement C1r Standard	1 vial
Sealing Tapes	3 units
Stop Solution	1 x 12ml

Research areas
Function

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.
Sequence similarities

Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 1 EGF-like domain.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains.
Post-translational
modifications

The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
Target information above from: UniProt accession P00736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Alternative names
- C1R
- C1R_HUMAN
- Complement C1r subcomponent
- Complement C1r subcomponent light chain
- Complement component 1 r subcomponent
- Complement component 1 subcomponent r
see all
Database links
- Entrez Gene: 715 Human
- Omim: 613785 Human
- SwissProt: P00736 Human
- Unigene: 524224 Human

Our Abpromise guarantee covers the use of ab170245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Sandwich ELISA		Use at an assay dependent concentration.

ab170245 has not yet been referenced specifically in any publications.

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Human Complement C1r ELISA Kit (ab170245)