Key features and details
- Sensitivity: 0.00323 µg/ml
- Range: 0.00323 µg/ml - 0.8 µg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman Complement C2 ELISA Kit
See all C2 kits
Intra-assay Sample n Mean SD CV% Overall 5.7% Inter-assay Sample n Mean SD CV% Overall 10.1%
Sample typeCell culture supernatant, Saliva, Milk, Serum, Plasma, Cerebral Spinal Fluid
Assay typeSandwich (quantitative)
Sensitivity> 0.00323 µg/ml
Range0.003 µg/ml - 0.8 µg/ml
Assay time4h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Does not react with: Mouse, Rat, Rabbit, Dog, Pig
Abcam’s Complement C2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C2 in CSF, plasma, serum, saliva, and cell culture supernatants.
A Complement C2 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C2 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C2 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 70X Biotinylated Human Complement C2 Antibody 1 x 90µl Chromogen Substrate 1 x 8ml Complement C2 Microplate (12 x 8 well strips) 1 unit Complement C2 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml
FunctionComponent C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase.
Involvement in diseaseDefects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain.
- Information by UniProt
- C3/C5 convertase
ab154132 has been referenced in 1 publication.
- Boire A et al. Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis. Cell 168:1101-1113.e13 (2017). PubMed: 28283064