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  1. Link

    human-complement-c3-elisa-kit-ab108822.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Human Complement C3 ELISA Kit (ab108822)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (12)References (9)

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ab108822 Complement C3 Human
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.2 µg/ml
  • Range: 0.117 µg/ml - 7.5 µg/ml
  • Sample type: Milk, Plasma, Saliva, Serum
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human

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Overview

  • Product name

    Human Complement C3 ELISA Kit
    See all C3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.3%
  • Sample type

    Saliva, Milk, Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    = 0.2 µg/ml
  • Range

    0.117 µg/ml - 7.5 µg/ml
  • Recovery

    99 %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Complement C3 ELISA kit is a competitive immunoassay designed for the quantitative measurement of Complement C3 in human plasma and serum.


    A Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection protein is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement C3 captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    1X Biotinylated Human Complement C3 (Lyophilized) 1 vial
    20X Wash Buffer Concentrate 1 x 30ml
    Chromogen Substrate 1 x 7ml
    Complement C3 Microplate (12 x 8 well strips) 1 unit
    Complement C3 Standard (Lyophilized) 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Immunology
    • Innate Immunity
    • Complement
    • Alternative Pathway
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Complement ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood coagulation ELISA kits
  • Function

    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications

    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01024 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Acylation stimulating protein cleavage product
    • AHUS5
    • ARMD9
    • ASP
    • C3
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
    • c3 complement
    • C3adesArg
    • CO3_HUMAN
    • Complement C3
    • Complement C3 alpha chain
    • Complement C3b alpha' chain
    • Complement C3c alpha' chain fragment 1
    • Complement C3c alpha' chain fragment 2
    • Complement C3c alpha'' chain fragment 2
    • Complement C3d fragment
    • Complement C3dg fragment
    • Complement C3f fragment
    • Complement C3g fragment
    • Complement component 3
    • Complement factor 3
    • CPAMD1
    • HEL S 62p
    • omplement C3 beta chain
    see all
  • Database links

    • Entrez Gene: 718 Human
    • Omim: 120700 Human
    • SwissProt: P01024 Human
    • Unigene: 529053 Human

    Images

    • ab108822 Complement C3 Human
      ab108822 Complement C3 Human

      Compl. C3 measured in biological fluids showing quantity (micrograms) per mL of tested sample

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab108822.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (9)

    Publishing research using ab108822? Please let us know so that we can cite the reference in this datasheet.

    ab108822 has been referenced in 9 publications.

    • Guldiken N  et al. Serum transferrin as a biomarker of hepatocyte nuclear factor 4 alpha activity and hepatocyte function in liver diseases. BMC Med 19:39 (2021). PubMed: 33593348
    • Brahmajothi MV & Abou-Donia MB Monitoring from Battlefield to Bedside: Serum Repositories Help Identify Biomarkers, Perspectives on Mild Traumatic Brain Injury. Mil Med 185:197-204 (2020). PubMed: 32074362
    • Qu S  et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
    • Lee DH  et al. Identification of serum biomarkers for premature ovarian failure. Biochim Biophys Acta Proteins Proteom 1867:219-226 (2019). PubMed: 30597202
    • Boire A  et al. Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis. Cell 168:1101-1113.e13 (2017). PubMed: 28283064
    • McGuire JL  et al. The complement system, neuronal injury, and cognitive function in horizontally-acquired HIV-infected youth. J Neurovirol 22:823-830 (2016). ELISA ; Human . PubMed: 27273074
    • Sweigard JH  et al. Inhibition of the alternative complement pathway preserves photoreceptors after retinal injury. Sci Transl Med 7:297ra116 (2015). PubMed: 26203084
    • Natrajan MS  et al. Pioglitazone regulates myelin phagocytosis and multiple sclerosis monocytes. Ann Clin Transl Neurol 2:1071-84 (2015). ELISA ; Human . PubMed: 26734659
    • Hepponstall M  et al. Remote ischemic preconditioning (RIPC) modifies the plasma proteome in children undergoing repair of tetralogy of fallot: a randomized controlled trial. PLoS One 10:e0122778 (2015). PubMed: 25826479

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Question

    I prefer a direct test ELISA, with a secondary antibody that binds to the C3 and conjugated with an enzyme. Do you have something like this?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 17 2013

    Answer

    The first assay you asked about, ab108823, is that kind of assay (sandwich), but the secondary (detection) antibody is biotinylated, not conjugated to an enzyme. The enzyme (HRP) is conjugated to streptavidin, which binds to the biotin on the antibody.

    The ab108823 assay is very sensitive, and C3 concentrations in plasma are high compared to the C3 concentrations of the sample types it is recommended for: supernatants, saliva, milk and urine. The assay can be used with plasma samples but they will need to be diluted in order to be within the range of the standards. The average concentration of C3 in normal human plasma is about 1.5 mg/ml. The range of standards in ab108823 is 0.625 - 40 ng/ml. So, you will need to dilute your samples approximately 1/100,000 - 1/1000,000 to be within the range of the standards.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Jun 17 2013

    Question

    bought ab108823 by accident (wanted ab108822)
    has precious serum/plasma samples and wants to know if she can use this kit with those
    has kit ab108823 been tested with serum/plasma samples?
    Why are there 2 separate kits and how are they different?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 19 2013

    Answer


    Complement C3 is very abundant in plasma/serum samples. We have developed two different assays.

    ab108823 is a sensitive sandwich ELISA assay. If you use this kit for plasma or serum sample, it requires very large dilution. Normal plasma C3 is average about 900 ug/ml. Our highest standard for ab108823 is 40 ng/ml. It requires a lot of dilution in order for sample to fall within the standard curve for kit ab108823. The more dilution, the less accurate it is for your assay and the higher the variation. That is why we develop the less sensitive assay ab108822 to address this issue.

    ab108822 reduces the dilution factor significantly. The assay is more reproducible than ab108823 for plasma.

    Read More

    Abcam Scientific Support

    Answered on Feb 19 2013

    Question

    i am using samples (not human) with very low concentrations of complement. I understand your kit was tested for human, but do you think I can use this undiluted or will the background be too high?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 16 2013

    Answer

    Thank you for contacting us. The laboratory does not think the background will be a problem with not diluting the samples. However, they did indicate that ab108823 may be more sensitive for detecting other species.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 16 2013

    Question

    Dear Sir,
    Thank you very much for your reply sir.
    We are using (ab 108823) Complement C3 Human ELISA kit for analysing the serum sample. We did the dilutions up to 1:40,000; but it shows the high absorbance. In the Kit, the protocol given in assay summary and assay method is different and yesterday you are sending the Kit Manual, it given different protocol, what we follow actually.
    A. General Information
    Lot number: GR 90621 – 1.
    Purchase order number/ order date or preferably the Abcam order number:
    Answer: We ordered last month October 2012 – We received on 3.11.12 and used from 4.11.12.
    - How was the kit stored?
    Answer: Based on the instructions given in the manual, we stored the kit.

    B. Description of the problem
    - Are the signals you obtained with control or standard satisfactory? (Yes/No);
    Answer: For standards we get the correct linearity standard graph, but for the samples, we did the serial dilutions.
    Serum direct = 3.0531,
    Serum 1: 100 dilution = 2.9485
    Serum 1: 200 dilution = 2.9846
    Serum 1:10,000 dilution = 1.7625
    Serum 1: 20,000 dilution = 1.6696.


    C. What do you think the problem is caused by? E.g. enzyme is faulty, developer is faulty or other component that you think might be faulty.
    Kindly help us to solve the problem.please trouble shoot us.
    Thank you

    Read More

    Abcam community

    Verified customer

    Asked on Nov 07 2012

    Answer

    I am sorry to hear that you have experienced so many problems with the Complement C3 Human ELISA kit (ab108823). I appreciate the time you took to provide further details.

    After reviewing the provided information I suggest to verify the samples type that was used with the kit.

    We offer two different Kits for Complement C3 Human. One can be used for cell culture supernatant, saliva, milk and urine. That is the ab108823 that was used in the experiments. The second kit is ab108822. This is made for the use with serum and plasma samples. The protocol and preparation of samples is different as well as the preparation of the standard curve to analyse the results.

    However, I contacted the laboratory for you and can confirm that the ab108823 can be used with plasma and serum, but the samples need to be diluted.

    It would require at least a 14000x dilution just to get a low range sample to within the upper range of the curve. And a 60000x or 120000x dilution to get it mid-curve.

    I hope this information is helpful for you. I wish you all the best luck for future experiments. If you continue to have any difficulties please let me know.

    Read More

    Abcam Scientific Support

    Answered on Nov 13 2012

    Question

    Dear sir,
    Sir, regarding Human Complement C3 ELISA kit, sir, we are having a query, we did complement C3 in Human serum, we did up to 1:20,000 dilution till we getting the ranges so high,please clarify it what dilutions its prefer for C3 in serum samples, in kit it didn't given.Kindly tell us sir.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 06 2012

    Answer

    Thank you for contacting us.

    I can confirm that our Complement C3 Human ELISA kit (ab108822) is tested to work with human serum and plasma and covered by our 6 month guarantee in case it does not work. Could you confirm if that is the kit you are using?

    We would not expect a high background for such high dilutions like 1:20000 because the recommended dilution for serum samples is 1:800 into diluent. The recommended dilution is stated in the protocol booklet. To read the whole protocol booklet click https://www.abcam.com/ps/products/108/ab108822/documents/ab108822%20Complement%20C3%20Human%20ELISA%20Kit%20v2%20(website).pdf.

    We take problems with our products very seriously, and investigate every product that we feel may not be performing correctly. I would like to investigate the problem further. I would appreciate if you could take some time to answer our questionnaire. Could you also please send me an example of your results to give me some more insight into the problem?

    A. General Information
    - Catalogue number:

    - Lot number:

    - Purchase order number/ order date or preferably the Abcam order number:

    - How was the kit stored?


    B. Description of the problem
    - Are the signals you obtained with control or standard satisfactory? (Yes/No);


    - Are the sample readings as expected or inconsistent?


    - Are the blank readings as expected?

    - Other (e.g. missing components)


    C. What do you think the problem is caused by? E.g. enzyme is faulty, developer is faulty or other component that you think might be faulty.


    Thank you for your cooperation. I look forward hearing from you again and hope I can help you after receiving more information.


    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 06 2012

    Question

    Estimada ,
    Gracias por tu asistencia. Nos ha sido de gran ayuda y ahora estamos analizando la información que tenemos para decidir qué ELISAs utilizar
    Atentamente

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2012

    Answer

    Muchas gracias por tu respuesta.

    Actualmente tenemos una promoción para España por la que se aplica el 25% de descuento a todos los productos que no son anticuerpos primarios. La información sobre la promoción está disponible en el siguiente link:

    https://www.abcam.com/index.html?pageconfig=resource&rid=15194

    Si necesitáis que os mandemos una proforma o cualquier información adicional, no dudéis en contactarnos.

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2012

    Question

    Estimad@ Sr/Sra,
    Necesitaría saber si los siguientes ELISAS humanos cross-reaccionan con el ortólogo murino:
    ab133037

    ab108822

    ab108823

    ab119524

    ab108848

    ab108847

    ab100582

    ab100582

    ab119612

    ab119611


    Y el “PLTP Activity Fluorometric Assay Kit” (ab65386)?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 03 2012

    Answer

    Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

    El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apropiado para vuestro propósito puesto que reacciona con el ortólogo murino.

    Para LIF Human ELISA Kit (ab100582) no se han llevado a cabo tests de reactividad cruzada, por lo que se desconoce si reconoce la proteína murina. El anticuerpo de captura es monoclonal y el de detección policlonal. Ambas proteínas comparten un 78% de homología, por lo que algo de reactividad cruzada es de esperar.

    Respecto a Complement C3 Human ELISA kit y Fibronectin Human ELISA Kit:

    ab108823: se ha observado una reactividad cruzada del 0.5% con ratón y del 10% con rata.

    ab108822: ninguna reactividad cruzada observada.

    ab108847: no se ha observado reactividad cruzada con el ortólogo murino

    ab108848: reactividad del 5% con ratón y del 20% con rata.

    Con Fibronectin Human ELISA kit (with color giving dyes) (ab119524) se ha encontrado cierta reactividad cruzada de acuerdo con el colaborador que nos lo suministra.

    Para ab119611 y ab119612 (uPA Human ELISA kit y uPA Receptor Human ELISA Kit) no se ha encontrado reactividad con la proteína de ratón.

    No tengo de momento información acerca de ab133037 pero si sigues interesado, en cuanto la tenga te contacto de nuevo.

    Si necesitáis un presupuesto o más información acerca de cualquiera de las opciones ofrecidas, por favor, no dudéis en contactarnos de nuevo y estaré encantada de poder ayudar.

    Read More

    Abcam Scientific Support

    Answered on Sep 03 2012

    Question

    Buenos días,

    Necesitaría información sobre los kits para la determinación de la actividad ASP en humanos: coste, nº de kits, coste envío… etc así como también si existe un manual que explique como utilizar.

    Reciban un cordial saludo

    Read More

    Abcam community

    Verified customer

    Asked on Jul 09 2012

    Answer

    Gracias por contactar con abcam.

    Me gustaría informarle que tenemos 2 kits para la deteccion de humano C3 (tambien llamado ASP).

    Las referencias sonab108822 (para Serum, Plasma) and ab108823 (para Cell culture supernatant, Saliva, Milk, Urine).

    Los links a las hojas de datos y a los protocoloslos encontrara a continuación:

    - ab108822:
    Datasheet: https://www.abcam.com/ab108822
    Protocol Booklet: https://www.abcam.com/ps/products/108/ab108822/documents/ab108822%20Complement%20C3%20Human%20ELISA%20Kit%20(Website).pdf

    - ab108823:
    Datasheet: https://www.abcam.com/ab108823
    Protocol booklet: https://www.abcam.com/ps/products/108/ab108823/documents/ab108823%20Complement%20C3%20Human%20ELISA%20Kit%20(Web)%20v2.pdf

    Informarle que los precios para los productos así como los gastos de envíoque aparecen ennuestra página web están totalmente actualizados.

    Esperamos que esta información le haya sido de utilidad. Por favor no dude en ponerse en contacto con nosotros si necesita algo mas.

    Reciba un cordial saludo.

    Read More

    Abcam Scientific Support

    Answered on Jul 09 2012

    Question

    Phone call requesting information on whether the kit is able to also detect C3a.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 05 2012

    Answer

    Thank you for contacting us in regards to Complement C3 Human ELISA kit (ab108822).


    I now have some further information to share with you concerning the specificity of the kit. The kit is able torecognize complement C3a and other isoforms of complement C3. The measurement made using this kit is to determine the total complement C3 present in the sample.


    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Abcam Scientific Support

    Answered on Mar 05 2012

    Question

    Phone call requesting clarification if a standard of concentration µg/mL as stated in protocol text or ng/mL as stated in the protocol table.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 02 2012

    Answer

    Thank you for contacting us and pointing out the inconsistency in the protocol provided with the Complement C3 Human ELISA kit (ab108822).


    I can confirm that the standard solutions should be made to ug/mL not ng/mL as state in the table of the protocol. This will be updated on the protocol sheet.


    I hope this information has been of help. If you require any further information please do not hesitate to contact us again. Otherwise, I wish you all the best with your research.

    Read More

    Abcam Scientific Support

    Answered on Feb 02 2012

    1-10 of 12 Abreviews or Q&A

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