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  1. Link

    human-complement-c3-elisa-kit-ab108823.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Human Complement C3 ELISA Kit (ab108823)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (2)Q&A (11)References (8)

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Human Complement C3 ELISA Kit
  • Sandwich ELISA - ab108823 Complement C3 Human ELISA kit

Key features and details

  • Sensitivity: 0.32 ng/ml
  • Range: 0.625 ng/ml - 40 ng/ml
  • Sample type: Cell culture supernatant, Cell Lysate, Cerebral Spinal Fluid, Milk, Saliva, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human Complement C3 ELISA Kit
    See all C3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.8%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Cell Lysate, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.32 ng/ml
  • Range

    0.625 ng/ml - 40 ng/ml
  • Recovery

    97 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Complement C3 ELISA kit is designed for the quantitative measurement of Complement C3 concentrations in Human urine, milk, saliva, cerebrospinal fluid and cell culture supernatants.


    A Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C3 captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    50X Biotinylated Human Complement C3 Antibody 1 x 120µl
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    Chromogen Substrate 1 x 7ml
    Complement C3 Microplate (12 x 8 well strips) 1 unit
    Complement C3 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Immunology
    • Innate Immunity
    • Complement
    • Alternative Pathway
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Complement ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood coagulation ELISA kits
  • Function

    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications

    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01024 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Acylation stimulating protein cleavage product
    • AHUS5
    • ARMD9
    • ASP
    • C3
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 1
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
    • c3 complement
    • C3adesArg
    • CO3_HUMAN
    • Complement C3
    • Complement C3 alpha chain
    • Complement C3b alpha' chain
    • Complement C3c alpha' chain fragment 1
    • Complement C3c alpha' chain fragment 2
    • Complement C3c alpha'' chain fragment 2
    • Complement C3d fragment
    • Complement C3dg fragment
    • Complement C3f fragment
    • Complement C3g fragment
    • Complement component 3
    • Complement factor 3
    • CPAMD1
    • HEL S 62p
    • omplement C3 beta chain
    see all
  • Database links

    • Entrez Gene: 718 Human
    • Omim: 120700 Human
    • SwissProt: P01024 Human
    • Unigene: 529053 Human

    Images

    • Human Complement C3 ELISA Kit
      Human Complement C3 ELISA Kit

      Representative Standard Curve using ab108823

    • Sandwich ELISA - ab108823 Complement C3 Human ELISA kit
      Sandwich ELISA - ab108823 Complement C3 Human ELISA kit

      Complement C3 measured in various samples showing quantity (microgram) per mL of tested sample

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (8)

    Publishing research using ab108823? Please let us know so that we can cite the reference in this datasheet.

    ab108823 has been referenced in 8 publications.

    • Kamitaki N  et al. Complement genes contribute sex-biased vulnerability in diverse disorders. Nature 582:577-581 (2020). PubMed: 32499649
    • Salazar-Camarena DC  et al. Analysis of the receptor BCMA as a biomarker in systemic lupus erythematosus patients. Sci Rep 10:6236 (2020). PubMed: 32277232
    • Chang TT  et al. Plasma proteome plus site-specific N-glycoprofiling for hepatobiliary carcinomas. J Pathol Clin Res 5:199-212 (2019). PubMed: 31136099
    • Pillai A  et al. Complement component 3 levels in the cerebrospinal fluid of cognitively intact elderly individuals with major depressive disorder. Biomark Neuropsychiatry 1:N/A (2019). PubMed: 31942568
    • El Saftawy EA  et al. The hidden impact of different Blastocystis genotypes on C-3 and IgE serum levels: a matter of debate in asthmatic Egyptian children. J Parasit Dis 43:443-451 (2019). PubMed: 31406409
    • Merchant ML  et al. Biomarker enhanced risk prediction for development of AKI after cardiac surgery. BMC Nephrol 19:102 (2018). PubMed: 29720115
    • Saini JS  et al. Nicotinamide Ameliorates Disease Phenotypes in a Human iPSC Model of Age-Related Macular Degeneration. Cell Stem Cell : (2017). PubMed: 28132833
    • McGuire JL  et al. The complement system, neuronal injury, and cognitive function in horizontally-acquired HIV-infected youth. J Neurovirol 22:823-830 (2016). ELISA ; Human . PubMed: 27273074

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 13 Abreviews or Q&A

    human C3 Elisa Kit

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    A good kit, works safely and reliably. We used the kit with cell supernatants. It's easy to handle compared to other Elisa kits and the instructions are easy to understand and follow.

    Abcam user community

    Verified customer

    Submitted Aug 30 2019

    good Elisa

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image
    The protocol booklet is well explained, easy to understand and easy to perform.
    I used cell culture supernatants.
    The kit is easy to use and quick compared to other Sandwich Elisa.
    We obtain reliable and reproducible results with this Elisa.

    Abcam user community

    Verified customer

    Submitted Feb 08 2018

    Question

    I prefer a direct test ELISA, with a secondary antibody that binds to the C3 and conjugated with an enzyme. Do you have something like this?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 17 2013

    Answer

    The first assay you asked about, ab108823, is that kind of assay (sandwich), but the secondary (detection) antibody is biotinylated, not conjugated to an enzyme. The enzyme (HRP) is conjugated to streptavidin, which binds to the biotin on the antibody.

    The ab108823 assay is very sensitive, and C3 concentrations in plasma are high compared to the C3 concentrations of the sample types it is recommended for: supernatants, saliva, milk and urine. The assay can be used with plasma samples but they will need to be diluted in order to be within the range of the standards. The average concentration of C3 in normal human plasma is about 1.5 mg/ml. The range of standards in ab108823 is 0.625 - 40 ng/ml. So, you will need to dilute your samples approximately 1/100,000 - 1/1000,000 to be within the range of the standards.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Jun 17 2013

    Question

    I would like to have one information about the Complement C3 Human ELISA kit: I noticed that the kit ab108823 isn't indicated for plasma, so I want to ask you if you have something for plasma.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 14 2013

    Answer

    A better choice for plasma samples is ab108822.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Jun 14 2013

    Question

    bought ab108823 by accident (wanted ab108822)
    has precious serum/plasma samples and wants to know if she can use this kit with those
    has kit ab108823 been tested with serum/plasma samples?
    Why are there 2 separate kits and how are they different?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 19 2013

    Answer


    Complement C3 is very abundant in plasma/serum samples. We have developed two different assays.

    ab108823 is a sensitive sandwich ELISA assay. If you use this kit for plasma or serum sample, it requires very large dilution. Normal plasma C3 is average about 900 ug/ml. Our highest standard for ab108823 is 40 ng/ml. It requires a lot of dilution in order for sample to fall within the standard curve for kit ab108823. The more dilution, the less accurate it is for your assay and the higher the variation. That is why we develop the less sensitive assay ab108822 to address this issue.

    ab108822 reduces the dilution factor significantly. The assay is more reproducible than ab108823 for plasma.

    Read More

    Abcam Scientific Support

    Answered on Feb 19 2013

    Question

    My collaborator bought some months ago different ELISA kits from your compagny. To understand our results, we need to known the sequence which is recognised by the antibodies used in these. Please can you send me this information for the following references:

    ab108884

    ab108823

    ab108883.

    Thanks in advance

    Read More

    Abcam community

    Verified customer

    Asked on Feb 11 2013

    Answer



    Unfortunately, I can confirm that the antibodies used in these kits have been raised against recombinant protein and purified proteins. The exact epitope of the antibodies have not been investigated further. We are therefore not able to provide you with any information as to the site of recognition involved.

    Read More

    Abcam Scientific Support

    Answered on Feb 11 2013

    Question

    Dear Sir,
    Thank you very much for your reply sir.
    We are using (ab 108823) Complement C3 Human ELISA kit for analysing the serum sample. We did the dilutions up to 1:40,000; but it shows the high absorbance. In the Kit, the protocol given in assay summary and assay method is different and yesterday you are sending the Kit Manual, it given different protocol, what we follow actually.
    A. General Information
    Lot number: GR 90621 – 1.
    Purchase order number/ order date or preferably the Abcam order number:
    Answer: We ordered last month October 2012 – We received on 3.11.12 and used from 4.11.12.
    - How was the kit stored?
    Answer: Based on the instructions given in the manual, we stored the kit.

    B. Description of the problem
    - Are the signals you obtained with control or standard satisfactory? (Yes/No);
    Answer: For standards we get the correct linearity standard graph, but for the samples, we did the serial dilutions.
    Serum direct = 3.0531,
    Serum 1: 100 dilution = 2.9485
    Serum 1: 200 dilution = 2.9846
    Serum 1:10,000 dilution = 1.7625
    Serum 1: 20,000 dilution = 1.6696.


    C. What do you think the problem is caused by? E.g. enzyme is faulty, developer is faulty or other component that you think might be faulty.
    Kindly help us to solve the problem.please trouble shoot us.
    Thank you

    Read More

    Abcam community

    Verified customer

    Asked on Nov 07 2012

    Answer

    I am sorry to hear that you have experienced so many problems with the Complement C3 Human ELISA kit (ab108823). I appreciate the time you took to provide further details.

    After reviewing the provided information I suggest to verify the samples type that was used with the kit.

    We offer two different Kits for Complement C3 Human. One can be used for cell culture supernatant, saliva, milk and urine. That is the ab108823 that was used in the experiments. The second kit is ab108822. This is made for the use with serum and plasma samples. The protocol and preparation of samples is different as well as the preparation of the standard curve to analyse the results.

    However, I contacted the laboratory for you and can confirm that the ab108823 can be used with plasma and serum, but the samples need to be diluted.

    It would require at least a 14000x dilution just to get a low range sample to within the upper range of the curve. And a 60000x or 120000x dilution to get it mid-curve.

    I hope this information is helpful for you. I wish you all the best luck for future experiments. If you continue to have any difficulties please let me know.

    Read More

    Abcam Scientific Support

    Answered on Nov 13 2012

    Question

    Customer kindly contacted us to inquire if this product will work with plasma.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 04 2012

    Answer

    Thank you for contacting us.

    I have been in touch with the lab to inquire aboout use ofab108823 Complement C3 Human ELISA kit with plasma.This kit can be used for plasma, but you need to find out the appropriate dilution factor. We suggest to start with a few series dilution to find out best dilution factor. For example, 1:1000. 1:10000, 1:100000 to find the best dilution for plasma.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 04 2012

    Question

    Estimada ,
    Gracias por tu asistencia. Nos ha sido de gran ayuda y ahora estamos analizando la información que tenemos para decidir qué ELISAs utilizar
    Atentamente

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2012

    Answer

    Muchas gracias por tu respuesta.

    Actualmente tenemos una promoción para España por la que se aplica el 25% de descuento a todos los productos que no son anticuerpos primarios. La información sobre la promoción está disponible en el siguiente link:

    https://www.abcam.com/index.html?pageconfig=resource&rid=15194

    Si necesitáis que os mandemos una proforma o cualquier información adicional, no dudéis en contactarnos.

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2012

    Question

    Estimad@ Sr/Sra,
    Necesitaría saber si los siguientes ELISAS humanos cross-reaccionan con el ortólogo murino:
    ab133037

    ab108822

    ab108823

    ab119524

    ab108848

    ab108847

    ab100582

    ab100582

    ab119612

    ab119611


    Y el “PLTP Activity Fluorometric Assay Kit” (ab65386)?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 03 2012

    Answer

    Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

    El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apropiado para vuestro propósito puesto que reacciona con el ortólogo murino.

    Para LIF Human ELISA Kit (ab100582) no se han llevado a cabo tests de reactividad cruzada, por lo que se desconoce si reconoce la proteína murina. El anticuerpo de captura es monoclonal y el de detección policlonal. Ambas proteínas comparten un 78% de homología, por lo que algo de reactividad cruzada es de esperar.

    Respecto a Complement C3 Human ELISA kit y Fibronectin Human ELISA Kit:

    ab108823: se ha observado una reactividad cruzada del 0.5% con ratón y del 10% con rata.

    ab108822: ninguna reactividad cruzada observada.

    ab108847: no se ha observado reactividad cruzada con el ortólogo murino

    ab108848: reactividad del 5% con ratón y del 20% con rata.

    Con Fibronectin Human ELISA kit (with color giving dyes) (ab119524) se ha encontrado cierta reactividad cruzada de acuerdo con el colaborador que nos lo suministra.

    Para ab119611 y ab119612 (uPA Human ELISA kit y uPA Receptor Human ELISA Kit) no se ha encontrado reactividad con la proteína de ratón.

    No tengo de momento información acerca de ab133037 pero si sigues interesado, en cuanto la tenga te contacto de nuevo.

    Si necesitáis un presupuesto o más información acerca de cualquiera de las opciones ofrecidas, por favor, no dudéis en contactarnos de nuevo y estaré encantada de poder ayudar.

    Read More

    Abcam Scientific Support

    Answered on Sep 03 2012

    1-10 of 13 Abreviews or Q&A

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