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  1. Link

    human-complement-c3a-des-arg-elisa-kit-ab133037.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Human Complement C3a des Arg ELISA Kit (ab133037)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (4)References (4)

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Typical Standard Curve

    Key features and details

    • Sensitivity: 0.12 ng/ml
    • Range: 0.313 ng/ml - 20 ng/ml
    • Sample type: Plasma
    • Detection method: Colorimetric
    • Assay type: Competitive
    • Reacts with: Human

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    Overview

    • Product name

      Human Complement C3a des Arg ELISA Kit
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Low conc. 16 1.86ng/ml 8.7%
      Medium conc. 16 3.4ng/ml 9.8%
      High conc. 16 12.3ng/ml 11.1%
      Inter-assay
      Sample n Mean SD CV%
      Low 0.753ng/ml 5.7%
      Medium 1.529ng/ml 16.9%
      High 3.491ng/ml 28.6%
    • Sample type

      Plasma
    • Assay type

      Competitive
    • Sensitivity

      0.12 ng/ml
    • Range

      0.313 ng/ml - 20 ng/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Plasma 94.8 % - %
    • Assay time

      3h 00m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Human
    • Product overview

      Abcam’s Complement C3a des Arg Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is for the accurate quantitation of Human Complement C3a des Arg in plasma.

      A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Complement C3a des Arg antigen and a polyclonal rabbit antibody specific to Complement C3a des Arg. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Complement C3a des Arg captured in the plate.

    • Notes

      The Human C3a des Arg molecule is one of three activation fragments formed from the activation of the complement cascade. C3a des Arg is formed from C3a via carboxypeptidase cleavage of the C-terminal arginine group. The structure of Human C3a des Arg was first reported in 1975 by Hugli. Human C3a des Arg contains 77 amino acids with 6 cysteines involved in disulfide bridges between residues 22-49, 23-56 and 36-57. The C terminal end of C3a des Arg in Human, porcine, rat, mouse and guinea pig is identical. C3a des Arg is a highly cationic molecule containing no carbohydrate. X-ray crystal data shows the N and C terminal residues to have highly flexible helical structures. C3a is one of the most potent constrictors of smooth muscle cells, and guinea pig airways are hyperresponsive to C3a when pretreated with histamine. The long term study of liver and other transplant recipients for both C3a des Arg and C4a des Arg may be useful in assessing a number of pathological conditions. The use of potent protease inhibitors, such as Futhan, in conjunction with EDTA, may allow complement activation factors to be quantitated specifically via inhibition of non-specific protease formation of C3a des Arg.

      Cross Reactivity

      CompoundCross Reactivity

      Human Complement C3a des Arg

      100%

      Human Complement C3

      1.28%

      Human Complement C4a des Arg

      0.40%
      Human Complement C4 0.31%

      Human Complement C5 des Arg

       0.10%
      Human Complement C5 0.05%

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer Concentrate 1 x 30ml
      Assay Buffer 10 Concentrate 1 x 15ml
      Complement Reagent A 1 x 15ml
      Complement Reagent B 1 x 30ml
      Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit
      Human Complement C3a des Arg EIA Antibody 1 x 6ml
      Human Complement C3a des Arg EIA Conjugate 1 x 6ml
      Human Complement C3a des Arg Standard 2 x 500ng
      Plate Sealer 2 units
      pNpp Substrate 1 x 23ml
      Stop Solution 1 x 5ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Complement
      • Classical Pathway
      • Immunology
      • Innate Immunity
      • Complement
      • Alternative Pathway
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Complement ELISA kits
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Blood coagulation ELISA kits
    • Relevance

      C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
    • Cellular localization

      Secreted
    • Alternative names

      • C3
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
      • Complement C3
      • CPAMD1
    • Database links

      • Entrez Gene: 718 Human
      • Omim: 120700 Human
      • SwissProt: P01024 Human

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative Standard Curve using ab133037

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (4)

      Publishing research using ab133037? Please let us know so that we can cite the reference in this datasheet.

      ab133037 has been referenced in 4 publications.

      • Zhu X  et al. All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-IL-1ß loop. Haematologica N/A:N/A (2019). PubMed: 30679324
      • Onwukwe C  et al. Engineering Intravenously Administered Nanoparticles to Reduce Infusion Reaction and Stop Bleeding in a Large Animal Model of Trauma. Bioconjug Chem 29:2436-2447 (2018). PubMed: 29965731
      • Hickman DA  et al. Intravenous synthetic platelet (SynthoPlate) nanoconstructs reduce bleeding and improve 'golden hour' survival in a porcine model of traumatic arterial hemorrhage. Sci Rep 8:3118 (2018). PubMed: 29449604
      • Zhang X & Sun L Anaphylatoxin C3a: A potential biomarker for esophageal cancer diagnosis. Mol Clin Oncol 8:315-319 (2018). PubMed: 29435296

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-4 of 4 Abreviews or Q&A

      Question

      Dear Sirs,



      We have a customer, who is looking for a Rat Complement C3a des Arg Elisa Kit and Abcam is offering a Kit with cat-nr. Ab133037. On the Datasheet there is the following sentence to be found:

      The C terminal end of C3a des Arg in Human, porcine, rat, mouse and guinea pig is identical

      Does this mean that the kit cross-reacts with these species?



      If not, would Abcam be willing to give a F.O.C-kit for the customer to evaluate the kit on the rat model? If it works, the customer would be glad to share the R&D-Information with Abcam and he would (in case the kit works) purchase 4 kits.



      I am looking forward in hearing from you.



      Thank you and kind regards,

      Read More

      Abcam community

      Verified customer

      Asked on Nov 12 2012

      Answer

      Thank you very much for your interest our products.

      I am sorry to confirm that we cannot recommend this kit for rat samples at the moment without concerns. I agree that the rat and human C3a des Arg are very alike, but this kit was not tested with rat and is optimized for human samples.

      My biggest concern here is that the standard is human C3a des Arg and that the kit is a competitive ELISA. This means that a difference in the binding efficiency in rat and human will lead to concentration information that might be not correct.

      We do have a program that is designed for our customers so that they can test our product in un-tested species:

      https://www.abcam.com/index.html?pageconfig=resource&rid=11998&source=pagetrap&viapagetrap=abtrial

      Please let your customer know that if they only want to compare different rat samples this kit might be suitable and that I am happy to offer the testing program if they think the kit would be suitable for their experiments.

      Read More

      Abcam Scientific Support

      Answered on Nov 12 2012

      Question

      I'm writing to answer if "Complement C3a des Arg, Human ELISA Kit - ab133037" can be also used with plasma citrate or only with plasma EDTA.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 08 2012

      Answer

      Thank you for your email.
      We recommend that plasma samples should be collected in EDTA/Futhan tubes in order to avoid possible low-level complement activation.
      If EDTA/Futhan tubes are not an option then EDTA could be used although EDTA/Futhan would be most optimal. Citrate does not have the inhibitory properties that EDTA and EDTA/Futhan; therefore, for this assay I would not recommend citrate.
      Please let me know if you have anymore questions.

      Read More

      Abcam Scientific Support

      Answered on Nov 08 2012

      Question

      Estimad@ Sr/Sra,
      Necesitaría saber si los siguientes ELISAS humanos cross-reaccionan con el ortólogo murino:

      ab133037

      ab108822

      ab108823

      ab119524

      ab108848

      ab108847

      ab100582

      ab100582

      ab119612

      ab119611

      Y el “PLTP Activity Fluorometric Assay Kit” (ab65386)?
      Muchas gracias

      Read More

      Abcam community

      Verified customer

      Asked on Sep 05 2012

      Answer

      Te confirmo también que este kit no se ha testado específicamente con muestras de ratón, pero esta diseñado específicamente para reconocer la proteína humana.

      Espero haber resuelto todas las dudas, y quedo a la espera de cualquier otra consulta que puedas tener.

      Read More

      Abcam Scientific Support

      Answered on Sep 05 2012

      Question

      Estimad@ Sr/Sra,
      Necesitaría saber si los siguientes ELISAS humanos cross-reaccionan con el ortólogo murino:
      ab133037

      ab108822

      ab108823

      ab119524

      ab108848

      ab108847

      ab100582

      ab100582

      ab119612

      ab119611


      Y el “PLTP Activity Fluorometric Assay Kit” (ab65386)?

      Read More

      Abcam community

      Verified customer

      Asked on Sep 03 2012

      Answer

      Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

      El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apropiado para vuestro propósito puesto que reacciona con el ortólogo murino.

      Para LIF Human ELISA Kit (ab100582) no se han llevado a cabo tests de reactividad cruzada, por lo que se desconoce si reconoce la proteína murina. El anticuerpo de captura es monoclonal y el de detección policlonal. Ambas proteínas comparten un 78% de homología, por lo que algo de reactividad cruzada es de esperar.

      Respecto a Complement C3 Human ELISA kit y Fibronectin Human ELISA Kit:

      ab108823: se ha observado una reactividad cruzada del 0.5% con ratón y del 10% con rata.

      ab108822: ninguna reactividad cruzada observada.

      ab108847: no se ha observado reactividad cruzada con el ortólogo murino

      ab108848: reactividad del 5% con ratón y del 20% con rata.

      Con Fibronectin Human ELISA kit (with color giving dyes) (ab119524) se ha encontrado cierta reactividad cruzada de acuerdo con el colaborador que nos lo suministra.

      Para ab119611 y ab119612 (uPA Human ELISA kit y uPA Receptor Human ELISA Kit) no se ha encontrado reactividad con la proteína de ratón.

      No tengo de momento información acerca de ab133037 pero si sigues interesado, en cuanto la tenga te contacto de nuevo.

      Si necesitáis un presupuesto o más información acerca de cualquiera de las opciones ofrecidas, por favor, no dudéis en contactarnos de nuevo y estaré encantada de poder ayudar.

      Read More

      Abcam Scientific Support

      Answered on Sep 03 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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