Overview

  • Product name

    Human Complement C3a des Arg ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low conc. 16 1.86ng/ml 8.7%
    Medium conc. 16 3.4ng/ml 9.8%
    High conc. 16 12.3ng/ml 11.1%
    Inter-assay
    Sample n Mean SD CV%
    Low 0.753ng/ml 5.7%
    Medium 1.529ng/ml 16.9%
    High 3.491ng/ml 28.6%
  • Sample type

    Plasma
  • Assay type

    Competitive
  • Sensitivity

    0.12 ng/ml
  • Range

    0.313 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Plasma 94.8 % - %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Complement C3a des Arg Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is for the accurate quantitation of Human Complement C3a des Arg in plasma.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Complement C3a des Arg antigen and a polyclonal rabbit antibody specific to Complement C3a des Arg. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Complement C3a des Arg captured in the plate.

  • Notes

    The Human C3a des Arg molecule is one of three activation fragments formed from the activation of the complement cascade. C3a des Arg is formed from C3a via carboxypeptidase cleavage of the C-terminal arginine group. The structure of Human C3a des Arg was first reported in 1975 by Hugli. Human C3a des Arg contains 77 amino acids with 6 cysteines involved in disulfide bridges between residues 22-49, 23-56 and 36-57. The C terminal end of C3a des Arg in Human, porcine, rat, mouse and guinea pig is identical. C3a des Arg is a highly cationic molecule containing no carbohydrate. X-ray crystal data shows the N and C terminal residues to have highly flexible helical structures. C3a is one of the most potent constrictors of smooth muscle cells, and guinea pig airways are hyperresponsive to C3a when pretreated with histamine. The long term study of liver and other transplant recipients for both C3a des Arg and C4a des Arg may be useful in assessing a number of pathological conditions. The use of potent protease inhibitors, such as Futhan, in conjunction with EDTA, may allow complement activation factors to be quantitated specifically via inhibition of non-specific protease formation of C3a des Arg.

    Cross Reactivity

    CompoundCross Reactivity

    Human Complement C3a des Arg

    100%

    Human Complement C3

    1.28%

    Human Complement C4a des Arg

    0.40%
    Human Complement C4 0.31%

    Human Complement C5 des Arg

     0.10%
    Human Complement C5 0.05%
  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 30ml
    Assay Buffer 10 Concentrate 1 x 15ml
    Complement Reagent A 1 x 15ml
    Complement Reagent B 1 x 30ml
    Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit
    Human Complement C3a des Arg EIA Antibody 1 x 6ml
    Human Complement C3a des Arg EIA Conjugate 1 x 6ml
    Human Complement C3a des Arg Standard 2 x 500ng
    Plate Sealer 2 units
    pNpp Substrate 1 x 23ml
    Stop Solution 1 x 5ml
  • Research areas

  • Relevance

    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Cellular localization

    Secreted
  • Alternative names

    • C3
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
    • Complement C3
    • CPAMD1
  • Database links

Applications

Our Abpromise guarantee covers the use of ab133037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab133037

Protocols

References

This product has been referenced in:

  • Zhu X  et al. All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-IL-1ß loop. Haematologica N/A:N/A (2019). Read more (PubMed: 30679324) »
  • Hickman DA  et al. Intravenous synthetic platelet (SynthoPlate) nanoconstructs reduce bleeding and improve 'golden hour' survival in a porcine model of traumatic arterial hemorrhage. Sci Rep 8:3118 (2018). Read more (PubMed: 29449604) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you very much for your interest our products.

I am sorry to confirm that we cannot recommend this kit for rat samples at the moment without concerns. I agree that the rat and human C3a des Arg are very alike, but this kit was not tested with rat and is optimized for human samples.

My biggest concern here is that the standard is human C3a des Arg and that the kit is a competitive ELISA. This means that a difference in the binding efficiency in rat and human will lead to concentration information that might be not correct.

We do have a program that is designed for our customers so that they can test our product in un-tested species:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&source=pagetrap&viapagetrap=abtrial

Please let your customer know that if they only want to compare different rat samples this kit might be suitable and that I am happy to offer the testing program if they think the kit would be suitable for their experiments.

Read More

Answer

Thank you for your email.
We recommend that plasma samples should be collected in EDTA/Futhan tubes in order to avoid possible low-level complement activation.
If EDTA/Futhan tubes are not an option then EDTA could be used although EDTA/Futhan would be most optimal. Citrate does not have the inhibitory properties that EDTA and EDTA/Futhan; therefore, for this assay I would not recommend citrate.
Please let me know if you have anymore questions.

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Answer

Te confirmo también que este kit no se ha testado específicamente con muestras de ratón, pero esta diseñado específicamente para reconocer la proteína humana.

Espero haber resuelto todas las dudas, y quedo a la espera de cualquier otra consulta que puedas tener.

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Answer

Siento no haber contestado antes, me temo que no tengo la información de todos los productos solicitados, sin embargo, puedo proporcionarte acerca de los siguientes kits:

El kit PLTP Activity Fluorometric Assay Kit (ab65386) no es apropiado para vuestro propósito puesto que reacciona con el ortólogo murino.

Para LIF Human ELISA Kit (ab100582) no se han llevado a cabo tests de reactividad cruzada, por lo que se desconoce si reconoce la proteína murina. El anticuerpo de captura es monoclonal y el de detección policlonal. Ambas proteínas comparten un 78% de homología, por lo que algo de reactividad cruzada es de esperar.

Respecto a Complement C3 Human ELISA kit y Fibronectin Human ELISA Kit:

ab108823: se ha observado una reactividad cruzada del 0.5% con ratón y del 10% con rata.

ab108822: ninguna reactividad cruzada observada.

ab108847: no se ha observado reactividad cruzada con el ortólogo murino

ab108848: reactividad del 5% con ratón y del 20% con rata.

Con Fibronectin Human ELISA kit (with color giving dyes) (ab119524) se ha encontrado cierta reactividad cruzada de acuerdo con el colaborador que nos lo suministra.

Para ab119611 y ab119612 (uPA Human ELISA kit y uPA Receptor Human ELISA Kit) no se ha encontrado reactividad con la proteína de ratón.

No tengo de momento información acerca de ab133037 pero si sigues interesado, en cuanto la tenga te contacto de nuevo.

Si necesitáis un presupuesto o más información acerca de cualquiera de las opciones ofrecidas, por favor, no dudéis en contactarnos de nuevo y estaré encantada de poder ayudar.

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