Human Complement C3a des Arg ELISA Kit (ab133037)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Human Complement C3a des Arg ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low conc. 16 1.86ng/ml 8.7% Medium conc. 16 3.4ng/ml 9.8% High conc. 16 12.3ng/ml 11.1% Inter-assay Sample n Mean SD CV% Low 0.753ng/ml 5.7% Medium 1.529ng/ml 16.9% High 3.491ng/ml 28.6% -
Sample type
Plasma -
Assay type
Competitive -
Sensitivity
0.12 ng/ml -
Range
0.313 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Plasma 94.8 % - % -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Complement C3a des Arg Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is for the accurate quantitation of Human Complement C3a des Arg in plasma.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Complement C3a des Arg antigen and a polyclonal rabbit antibody specific to Complement C3a des Arg. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Complement C3a des Arg captured in the plate.
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Notes
The Human C3a des Arg molecule is one of three activation fragments formed from the activation of the complement cascade. C3a des Arg is formed from C3a via carboxypeptidase cleavage of the C-terminal arginine group. The structure of Human C3a des Arg was first reported in 1975 by Hugli. Human C3a des Arg contains 77 amino acids with 6 cysteines involved in disulfide bridges between residues 22-49, 23-56 and 36-57. The C terminal end of C3a des Arg in Human, porcine, rat, mouse and guinea pig is identical. C3a des Arg is a highly cationic molecule containing no carbohydrate. X-ray crystal data shows the N and C terminal residues to have highly flexible helical structures. C3a is one of the most potent constrictors of smooth muscle cells, and guinea pig airways are hyperresponsive to C3a when pretreated with histamine. The long term study of liver and other transplant recipients for both C3a des Arg and C4a des Arg may be useful in assessing a number of pathological conditions. The use of potent protease inhibitors, such as Futhan, in conjunction with EDTA, may allow complement activation factors to be quantitated specifically via inhibition of non-specific protease formation of C3a des Arg.
Cross Reactivity
Compound Cross Reactivity Human Complement C3a des Arg
100% Human Complement C3
1.28% Human Complement C4a des Arg
0.40% Human Complement C4 0.31% Human Complement C5 des Arg
0.10% Human Complement C5 0.05% -
Tested applications
Suitable for: Competitive ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 30ml Assay Buffer 10 Concentrate 1 x 15ml Complement Reagent A 1 x 15ml Complement Reagent B 1 x 30ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Human Complement C3a des Arg EIA Antibody 1 x 6ml Human Complement C3a des Arg EIA Conjugate 1 x 6ml Human Complement C3a des Arg Standard 2 x 500ng Plate Sealer 2 units pNpp Substrate 1 x 23ml Stop Solution 1 x 5ml -
Research areas
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Relevance
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
Cellular localization
Secreted -
Alternative names
- C3
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
- Complement C3
- CPAMD1
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Database links
- Entrez Gene: 718 Human
- Omim: 120700 Human
- SwissProt: P01024 Human
Applications
Our Abpromise guarantee covers the use of ab133037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative Standard Curve using ab133037
Protocols
References
This product has been referenced in:
- Zhu X et al. All-trans retinoic acid protects mesenchymal stem cells from immune thrombocytopenia by regulating the complement-IL-1ß loop. Haematologica N/A:N/A (2019). Read more (PubMed: 30679324) »
- Hickman DA et al. Intravenous synthetic platelet (SynthoPlate) nanoconstructs reduce bleeding and improve 'golden hour' survival in a porcine model of traumatic arterial hemorrhage. Sci Rep 8:3118 (2018). Read more (PubMed: 29449604) »
