Overview

  • Product name

    Human Complement C4-Binding Protein ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.1%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.9 ng/ml
  • Range

    0.938 ng/ml - 60 ng/ml
  • Recovery

    97 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow, Dog, Pig, Monkey
  • Product overview

    ab222866 Human Complement C4-Binding Protein ELISA Kit is designed for the quantitative measurement of Complement C4-Binding Protein in plasma, serum, urine, milk, saliva, cerebrospinal fluid (CSF), and cell culture samples.


    The kit employs a quantitative sandwich enzyme immunoassay technique that measures human Complement C4-Binding Protein (C4BP) in less than 4 hours. A polyclonal antibody specific for human complement C4BP has been pre-coated onto a 96-well microplate with removable strips. Complement C4BP in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for complement C4BP, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Complement component C4-binding protein (C4BP) regulates the complement system by accelerating the decay of the complement component C3 convertase and by acting as a cofactor to the serine protease factor I in the degradation of C4b. C4BP is a high molecular mass (570 kDa) glycoprotein and is present in plasma in various isoforms with different alpha beta composition. The major form of C4BP is composed of seven identical 70-kDa alpha chains, each containing a binding site for the complement protein C4b, and a unique 45 kDa beta chain which contains a binding site for the vitamin K-dependent protein S. C4BP was overexpressed in the synovial membranes of patients with rheumatoid arthritis. It was detected in amyloid-beta plaques and on apoptotic cells.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    Anti- Human Complement C4BP coated Microplate (12 x 8 wells) 1 unit
    50X Biotinylated Human Complement C4BP 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Human Complement C4BP Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.
  • Sequence similarities

    Contains 3 Sushi (CCP/SCR) domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C4b binding protein beta chain
    • C4b-binding protein beta chain
    • C4BP
    • C4BPB
    • C4BPB_HUMAN
    • Complement component 4 binding protein beta
    • Complement component 4 binding protein beta chain
    • Complement component 4-binding protein beta
    • OTTHUMP00000034288
    • OTTHUMP00000034289
    • OTTHUMP00000034375
    • OTTHUMP0000003474
    • Proline rich protein
    • PRP
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of human Complement C4-Binding Protein standard curve.

Protocols

References

ab222866 has not yet been referenced specifically in any publications.

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