Overview

  • Product name
    Human Complement C4 ELISA Kit
    See all Complement C4 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.1%
  • Sample type
    Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.25 µg/ml
  • Range
    0.313 µg/ml - 5 µg/ml
  • Recovery

    94 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Complement C4 Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108834) is designed for the quantitative measurement of Complement C4 levels in plasma and serum.


    A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Complement C4 is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement C4 captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Biotinylated Human Complement C4 (Lyophilized) 1 vial
    20X Wash Buffer Concentrate 1 x 30ml
    Chromogen Substrate 1 x 8ml
    Complement C4 Microplate (12 x 8 well strips) 1 unit
    Complement C4 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.
    Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificity
    Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland,and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extra-hepatic sites of expression may be important for the local protection and inflammatory response.
  • Involvement in disease
    Complement component 4A deficiency
    Systemic lupus erythematosus
  • Sequence similarities
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications
    Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. The proteolytic cleavages often are incomplete so that many structural forms can be found in plasma.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • Acidic complement C4
    • Basic complement C4
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 2
    • C3 and PZP like alpha 2 macroglobulin domain containing protein 3
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
    • C4A
    • C4B
    • CO4
    • CO4A_HUMAN
    • Complement C4 A
    • Complement C4 B
    • Complement C4 gamma chain
    • Complement component 4A
    • Complement component 4B
    • CPAMD2
    • CPAMD3
    see all
  • Database links
    see all

Applications

Our Abpromise guarantee covers the use of ab108824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • ab108824 Complement C4 Human ELISA Kit
    ab108824 Complement C4 Human ELISA Kit

    Complement C4 measured in biological fluids showing quantity (microgram) per mL of tested sample

  • ab108824 Complement C4 Human ELISA Kit
    ab108824 Complement C4 Human ELISA Kit

    Standard curve : mean of duplicates (+/-SD)

Protocols

References

This product has been referenced in:
  • Owsley C  et al. Associations between abnormal rod-mediated dark adaptation and health and functioning in older adults with normal macular health. Invest Ophthalmol Vis Sci 55:4776-89 (2014). ELISA ; Human . Read more (PubMed: 24854857) »

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