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    human-complement-c4-elisa-kit-ab108824.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Human Complement C4 ELISA Kit (ab108824)

  • Datasheet
  • SDS
  • Protocol Booklet
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ab108824  	Complement C4 Human ELISA Kit
  • ab108824  Complement C4 Human ELISA Kit

Key features and details

  • Sensitivity: 0.25 µg/ml
  • Range: 0.313 µg/ml - 5 µg/ml
  • Sample type: Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human

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Overview

  • Product name

    Human Complement C4 ELISA Kit
    See all C4 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.1%
  • Sample type

    Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    = 0.25 µg/ml
  • Range

    0.313 µg/ml - 5 µg/ml
  • Recovery

    94 %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Complement C4 Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108834) is designed for the quantitative measurement of Complement C4 levels in plasma and serum.


    A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Complement C4 is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement C4 captured in plate.


    Reactivity with C4 cleavage products (C4a, C4b, C4d) has not been determined.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Biotinylated Human Complement C4 (Lyophilized) 1 vial
    20X Wash Buffer Concentrate 1 x 30ml
    Chromogen Substrate 1 x 8ml
    Complement C4 Microplate (12 x 8 well strips) 1 unit
    Complement C4 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Complement ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood coagulation ELISA kits
  • Target information above from: UniProt accession P0C0L4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Acidic C4
    • Acidic complement C4
    • Basic C4
    • Basic complement C4
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
    • C4, Chido form
    • C4, Rodgers from
    • C4A
    • C4A anaphylatoxin
    • C4A2
    • C4A3
    • C4A4
    • C4A6
    • C4AD
    • C4B
    • C4B_2
    • C4B1
    • C4B12
    • C4B2
    • C4B3
    • C4BD
    • C4F
    • C4S
    • CH
    • Chido form of C4
    • CO4
    • CO4A_HUMAN
    • Complement C4 A
    • Complement C4 B
    • Complement C4 gamma chain
    • complement C4-A
    • complement C4-B
    • complement C4-B-like
    • complement C4B1a
    • Complement component 4A
    • Complement component 4A (Rodgers blood group)
    • Complement component 4B
    • complement component 4B (Chido blood group)
    • complement component 4B (Chido blood group), copy 2
    • Complement component 4B (Childo blood group)
    • Complement component 4F
    • Complement component 4S
    • CPAMD2
    • CPAMD3
    • RG
    • Rodgers form of C4
    see all
  • Database links

    • Entrez Gene: 720 Human
    • Entrez Gene: 721 Human
    • Entrez Gene: 100293534 Human
    • Entrez Gene: 721 Human
    • Omim: 120810 Human
    • Omim: 120820 Human
    • SwissProt: P0C0L4 Human
    • SwissProt: P0C0L5 Human
    • Unigene: 534847 Human
    • Unigene: 720022 Human
    • Unigene: 534847 Human
    • Unigene: 720022 Human
    see all

Images

  • ab108824  	Complement C4 Human ELISA Kit
    ab108824 Complement C4 Human ELISA Kit

    Complement C4 measured in biological fluids showing quantity (microgram) per mL of tested sample

  • ab108824  Complement C4 Human ELISA Kit
    ab108824 Complement C4 Human ELISA Kit

    Standard curve : mean of duplicates (+/-SD)

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (7)

Publishing research using ab108824? Please let us know so that we can cite the reference in this datasheet.

ab108824 has been referenced in 7 publications.

  • Qu S  et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
  • Martin M  et al. Plasma C4d Correlates With C4d Deposition in Kidneys and With Treatment Response in Lupus Nephritis Patients. Front Immunol 11:582737 (2020). PubMed: 33133102
  • Salazar-Camarena DC  et al. Analysis of the receptor BCMA as a biomarker in systemic lupus erythematosus patients. Sci Rep 10:6236 (2020). PubMed: 32277232
  • Cleary SJ  et al. Complement activation on endothelium initiates antibody-mediated acute lung injury. J Clin Invest 130:5909-5923 (2020). PubMed: 32730229
  • Walss-Bass C  et al. X-Aptamer Technology Identifies C4A and ApoB in Blood as Potential Markers for Schizophrenia. Mol Neuropsychiatry 5:52-59 (2019). PubMed: 31019918
  • Liu Y  et al. Toll-like receptor-9 is involved in the development of B cell stimulating factor-induced systemic lupus erythematosus. Exp Ther Med 15:585-591 (2018). PubMed: 29387207
  • Owsley C  et al. Associations between abnormal rod-mediated dark adaptation and health and functioning in older adults with normal macular health. Invest Ophthalmol Vis Sci 55:4776-89 (2014). ELISA ; Human . PubMed: 24854857

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