Human Complement C4 ELISA Kit (ab108824)
Key features and details
- Sensitivity: 0.25 µg/ml
- Range: 0.313 µg/ml - 5 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Complement C4 ELISA Kit
See all C4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3.6% Inter-assay Sample n Mean SD CV% Overall 9.1% -
Sample type
Serum, Plasma -
Assay type
Competitive -
Sensitivity
= 0.25 µg/ml -
Range
0.313 µg/ml - 5 µg/ml -
Recovery
94 %
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Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Complement C4 Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108834) is designed for the quantitative measurement of Complement C4 levels in plasma and serum.
A Complement C4 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Complement C4 is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement C4 captured in plate.
Reactivity with C4 cleavage products (C4a, C4b, C4d) has not been determined.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Biotinylated Human Complement C4 (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Chromogen Substrate 1 x 8ml Complement C4 Microplate (12 x 8 well strips) 1 unit Complement C4 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
- Information by UniProt
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Alternative names
- Acidic C4
- Acidic complement C4
- Basic C4
see all -
Database links
- Entrez Gene: 720 Human
- Entrez Gene: 721 Human
- Entrez Gene: 100293534 Human
- Entrez Gene: 721 Human
- Omim: 120810 Human
- Omim: 120820 Human
- SwissProt: P0C0L4 Human
- SwissProt: P0C0L5 Human
see all
Images
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ab108824 Complement C4 Human ELISA Kit
Complement C4 measured in biological fluids showing quantity (microgram) per mL of tested sample
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ab108824 Complement C4 Human ELISA KitStandard curve : mean of duplicates (+/-SD)
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab108824 has been referenced in 7 publications.
- Qu S et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
- Martin M et al. Plasma C4d Correlates With C4d Deposition in Kidneys and With Treatment Response in Lupus Nephritis Patients. Front Immunol 11:582737 (2020). PubMed: 33133102
- Salazar-Camarena DC et al. Analysis of the receptor BCMA as a biomarker in systemic lupus erythematosus patients. Sci Rep 10:6236 (2020). PubMed: 32277232
- Cleary SJ et al. Complement activation on endothelium initiates antibody-mediated acute lung injury. J Clin Invest 130:5909-5923 (2020). PubMed: 32730229
- Walss-Bass C et al. X-Aptamer Technology Identifies C4A and ApoB in Blood as Potential Markers for Schizophrenia. Mol Neuropsychiatry 5:52-59 (2019). PubMed: 31019918
- Liu Y et al. Toll-like receptor-9 is involved in the development of B cell stimulating factor-induced systemic lupus erythematosus. Exp Ther Med 15:585-591 (2018). PubMed: 29387207
- Owsley C et al. Associations between abnormal rod-mediated dark adaptation and health and functioning in older adults with normal macular health. Invest Ophthalmol Vis Sci 55:4776-89 (2014). ELISA ; Human . PubMed: 24854857