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    human-complement-c5-elisa-kit-ab125963.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Human Complement C5 ELISA Kit (ab125963)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Q&A (2)References (6)

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Sandwich ELISA - Complement C5 Human ELISA Kit (ab125963)
  • Typical Stand Curve

Key features and details

  • Sensitivity: 68 pg/ml
  • Range: 0.156 ng/ml - 10 ng/ml
  • Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human Complement C5 ELISA Kit
    See all C5 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.8%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    68 pg/ml
  • Range

    0.156 ng/ml - 10 ng/ml
  • Recovery

    101 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Complement C5 ELISA kit (Enzyme-Linked Immunosorbent Assay) is designed for the quantitative measurement of C5 in human plasma, serum, saliva, milk, CSF and cell culture supernatants.


    A Complement C5 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C5 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C5 captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Complement C5 Antibody 1 x 120µl
    Chromogen Substrate 1 x 7ml
    Complement C5 Microplate (12 x 8 well strips) 1 unit
    Complement C5 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Immunology
    • Innate Immunity
    • Complement
    • Alternative Pathway
    • Immunology
    • Innate Immunity
    • Complement
    • MAC
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Complement ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Blood coagulation ELISA kits
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
  • Function

    Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • Involvement in disease

    Defects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01031 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Anaphylatoxin C5a analog
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
    • C5
    • C5a
    • C5a anaphylatoxin
    • C5b
    • CO5_HUMAN
    • Complement C5
    • Complement C5 alpha'' chain
    • Complement component C5
    • CPAMD4
    • prepro-C5
    see all
  • Database links

    • Entrez Gene: 727 Human
    • Omim: 120900 Human
    • SwissProt: P01031 Human
    • Unigene: 494997 Human

    Images

    • Sandwich ELISA - Complement C5 Human ELISA Kit (ab125963)
      Sandwich ELISA - Complement C5 Human ELISA Kit (ab125963)
      Complement 5 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
    • Typical Stand Curve
      Typical Stand Curve

      Representative Standard Curve using ab125963

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab125963? Please let us know so that we can cite the reference in this datasheet.

    ab125963 has been referenced in 6 publications.

    • Latuszek A  et al. Inhibition of complement pathway activation with Pozelimab, a fully human antibody to complement component C5. PLoS One 15:e0231892 (2020). PubMed: 32384086
    • Meng X  et al. DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma. EBioMedicine 41:185-199 (2019). PubMed: 30773478
    • Kusner LL  et al. Investigational RNAi Therapeutic Targeting C5 Is Efficacious in Pre-clinical Models of Myasthenia Gravis. Mol Ther Methods Clin Dev 13:484-492 (2019). PubMed: 31193726
    • Csosz É  et al. Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population. FEBS Open Bio 8:556-569 (2018). PubMed: 29632809
    • Guerra AD  et al. Minocycline modulates NF?B phosphorylation and enhances antimicrobial activity against Staphylococcus aureus in mesenchymal stromal/stem cells. Stem Cell Res Ther 8:171 (2017). ELISA ; Human . PubMed: 28732530
    • Owsley C  et al. Associations between abnormal rod-mediated dark adaptation and health and functioning in older adults with normal macular health. Invest Ophthalmol Vis Sci 55:4776-89 (2014). ELISA ; Human . PubMed: 24854857

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I have a question in reference to your C5 kit (ab125963). Specifically, I am looking for an assay that detects only unactivated C5, not C5a or C5a-des-Arg. I was not clear from your product information if your ELISA measures both C5 and C5a or just the unactivated form. Can you clarify this for me?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 21 2012

    Answer

    Thank you for contacting Abcam regarding ab125963.


    I have confirmed that this kit will react withboth C5 and C5a.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Read More

    Abcam Scientific Support

    Answered on Aug 21 2012

    Question

    Is the protein used as standard from a native source or rather recombinant?
    What is the MW of the protein standard?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 03 2012

    Answer

    Thank you for contacting us.

    The kit standard is purified from human plasma with >95% purity by SDS-PAGE. The MW is 190,000 Da (2 chains).

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Aug 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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