Overview

  • Product name
    Human Complement C5 ELISA Kit
    See all Complement C5 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 12.2%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    0.1 ng/ml
  • Range
    0.313 ng/ml - 20 ng/ml
  • Recovery

    101 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Complement C5 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab125963) is designed for the quantitative measurement of C5 in human plasma, serum, saliva, milk, CSF and cell culture supernatants.


    A Complement C5 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C5 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C5 captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Complement C5 Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Complement C5 Microplate (12 x 8 well strips) 1 unit
    Complement C5 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Function
    Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
    Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis).
  • Involvement in disease
    Defects in C5 are the cause of complement component 5 deficiency (C5D) [MIM:609536]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
    Note=An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
  • Sequence similarities
    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Alternative names
    • anaphylatoxin C5a analog
    • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
    • C5
    • CO5_HUMAN
    • complement C5
    • Complement C5 alpha'' chain
    • Complement component 5
    • CPAMD4
    • ECLZB
    see all
  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab125963 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Sandwich ELISA - Complement C5 Human ELISA Kit (ab125963)
      Sandwich ELISA - Complement C5 Human ELISA Kit (ab125963)
      Complement 5 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
    • Typical Stand Curve
      Typical Stand Curve

      Representative Standard Curve using ab125963

    Protocols

    References

    This product has been referenced in:
    • Csosz É  et al. Salivary proteome profiling of oral squamous cell carcinoma in a Hungarian population. FEBS Open Bio 8:556-569 (2018). Read more (PubMed: 29632809) »
    • Guerra AD  et al. Minocycline modulates NF?B phosphorylation and enhances antimicrobial activity against Staphylococcus aureus in mesenchymal stromal/stem cells. Stem Cell Res Ther 8:171 (2017). ELISA ; Human . Read more (PubMed: 28732530) »

    See all 3 Publications for this product

    Publishing research using ab125963? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    2 reviews or Q&A

    10
    Votes: Highest First

    I have a question in reference to your C5 kit (ab125963). Specifically, I am looking for an assay that detects only unactivated C5, not C5a or C5a-des-Arg. I was not clear from your product information if your ELISA measures both C5 and C5a or just the...

    Read More

    Thank you for contacting Abcam regarding ab125963.


    I have confirmed that this kit will react withboth C5 and C5a.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

    Is the protein used as standard from a native source or rather recombinant?
    What is the MW of the protein standard?

    Thank you for contacting us.

    The kit standard is purified from human plasma with >95% purity by SDS-PAGE. The MW is 190,000 Da (2 chains).

    I hope this information is helpful to you. Please do not hesitate to contact us if you ne...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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