Overview

  • Product name

    Human Complement C8 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.3%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.6 ng/ml
  • Range

    0.625 ng/ml - 40 ng/ml
  • Recovery

    = 98 %

    Sample specific recovery
    Sample type Average % Range
    Milk 92% - 103%
    Serum 94% - 107%
    Plasma 96% - 106%

  • Assay time

    4h 0m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Complement C8 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement C8 in Human plasma, serum, saliva, milk, and cell culture supernatants.

    A Complement C8 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C8 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Complement C8 captured in plate.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    50X Biotinylated Human Complement C8 Antibody 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Complement C8 Microplate (12 x 8 well strips) 1 unit
    Complement C8 Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Function

    Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C8A inserts into the target membrane, but does not form pores by itself.
  • Involvement in disease

    Defects in C8A are a cause of complement component 8 deficiency type 1 (C8D1) [MIM:613790]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
  • Sequence similarities

    Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 EGF-like domain.
    Contains 1 LDL-receptor class A domain.
    Contains 1 MACPF domain.
    Contains 2 TSP type-1 domains.
  • Cellular localization

    Secreted. Cell membrane. Secreted as soluble protein. Inserts into the cell membrane of target cells.
  • Information by UniProt
  • Alternative names

    • C8A
    • CO8A_HUMAN
    • Complement component 8 subunit alpha
    • Complement component C8 alpha chain
  • Database links

Applications

Our Abpromise guarantee covers the use of ab137971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab137971

Protocols

References

ab137971 has not yet been referenced specifically in any publications.

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