Key features and details
- Sensitivity: 0.3 µg/ml
- Range: 0.375 µg/ml - 24 µg/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Product nameHuman Complement Factor I ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 7.3% Inter-assay Sample n Mean SD CV% Overall 10.1%
Sample typeCell culture supernatant, Saliva, Milk, Serum, Plasma
Range0.375 µg/ml - 24 µg/ml
Assay time3h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Complement Factor I Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Complement Factor I levels in plasma, serum, milk, saliva, and cell culture supernatant.
A Complement Factor I specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Complement Factor I is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement Factor I captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml Biotinylated Complement Factor I (Lyophilised) 1 vial Chromogen Substrate 1 x 8ml Complement Factor I Microplate (12 x 8 well strips) 1 unit Complement Factor I Standard (Lyophilised) 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml
FunctionResponsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.
Involvement in diseaseHemolytic uremic syndrome atypical 3
Complement factor I deficiency
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 Kazal-like domain.
Contains 2 LDL-receptor class A domains.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- C3b INA
ab195460 has been referenced in 2 publications.