Overview

  • Product name

    Human Complement Factor P ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 1 20 5.1%
    Plasma 2 20 4.8%
    Plasma 3 20 5%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 1 20 9.9%
    Plasma 2 20 9.7%
    Plasma 3 20 10.2%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Plasma, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.1 ng/ml
  • Range

    0.156 ng/ml - 10 ng/ml
  • Recovery

    96 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • Product overview

    The Human Complement Factor P (CFP) ELISA (Enzyme-Linked Immunosorbent Assay) Kit (ab222864) is designed for detection of Complement Factor P in human plasma, serum, urine, saliva, CSF, and cell culture samples.


    This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Complement Factor P in approximately 4 hours. A polyclonal antibody specific for human Complement Factor P has been pre-coated onto a 96-well microplate with removable strips. Complement Factor P in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human Complement Factor P, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Complement factor P (CFP), also known as properdin, is a plasma glycoprotein that positively regulates the alternative complement pathway convertases (C3bBb) of the innate immune system. CFP consists of 442-amino acids with a molecular weight of 53 kDa and is composed of multiple identical protein subunits. The subunits bind to each other in a head to tail manner to form cyclic dimers, trimers, tetramers, pentamers, and higher cyclic oligomers. It is mainly produced by neutrophils but also by monocytes, T cells, and bone marrow progenitor cells. CFP binds to many microbial surfaces and apoptotic cells. Complement factor P also stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    Anti-Human Complement Factor P coated Microplate (12 x 8 wells) 1 unit
    50X Biotinylated Human Complement Factor P 1 x 120µl
    Chromogen Substrate 1 x 8ml
    Human Complement Factor P Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas

  • Relevance

    Factor P (Properdin) is a plasma protein that is active in the alternative complement pathway of the innate immune system. It is a positive regulatory factor that binds to many microbial surfaces to stabilize the C3b,Bb convertase. The C3b,Bb convertase then rapidly cleaves more C3 to C3b, which acts either as an opsonin or to reinitiate the pathway in an amplification loop that proceeds on the bacterial cell, but not on the host cell. In the alternative pathway, C3 is thus activated through factor B, factor D, and properdin P, under the control of factors I and H. Deficiency of properdin is associated in particular with a heightened susceptibility to Neisseria species.
  • Cellular localization

    Secreted
  • Alternative names

    • CFP
    • Complement factor P
    • PFC
    • Properdin
  • Database links

Applications

Our Abpromise guarantee covers the use of ab222864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Example of human Complement Factor P standard curve in 1X Diluent M. The standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab222864 has not yet been referenced specifically in any publications.

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