Overview

  • Product name

    Human Coronin 1a ELISA Kit
    See all Coronin 1a/TACO kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Human serum 5 5.6%
    Inter-assay
    Sample n Mean SD CV%
    Human serum 3 6.4%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    49 pg/ml
  • Range

    0.313 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 93 89% - 98%
    Cell culture extracts 102 80% - 120%
    Heparin Plasma 96 93% - 98%
    EDTA Plasma 93 89% - 94%
    Citrate Plasma 83 79% - 87%
    serum free media 94 88% - 98%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Coronin 1a in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Coronin 1a protein in serum, plasma, cell culture supernatant, and cell and tissue extract.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples in Sample Diluent NS: 49 pg/mL
    Samples in 1X Cell Extraction Buffer PTR: 80 pg/mL

  • Notes

    Coronin 1A is a WD-repeat containing protein that plays a role in cytoskeleton dynamics. Coronin 1A binds to actin. Mouse and rat Coronin 1A have 95% and 93% protein sequence identity to human Coronin 1A.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Coronin 1a Capture Antibody 1 x 600µl
    10X Human Coronin 1a Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    Human Coronin 1a Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
  • Tissue specificity

    Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
  • Sequence similarities

    Belongs to the WD repeat coronin family.
    Contains 7 WD repeats.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.
  • Information by UniProt
  • Alternative names

    • Actin binding protein
    • CLABP
    • Clipin A
    • Clipin-A
    • CLIPINA
    • COR1A_HUMAN
    • CORO1A
    • Coronin 1
    • Coronin actin binding protein 1A
    • Coronin like protein A
    • Coronin like protein p57
    • Coronin-1A
    • Coronin-like protein A
    • Coronin-like protein p57
    • FLJ41407
    • HCORO1
    • MGC117380
    • OTTHUMP00000163017
    • p57
    • TACO
    • Tryptophan aspartate containing coat protein
    • Tryptophan aspartate-containing coat protein
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab214032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Coronin 1a were measured in duplicates, interpolated from the Coronin 1a standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (EDTA) 5%, plasma (heparin) 5% and plasma (citrate) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Coronin 1a concentration was determined to be 37.4 ng/mL in serum, 34.3 ng/mL in plasma (EDTA), 18.7 ng/mL in plasma (heparin) and 33.6 ng/mL in plasma (citrate).

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Coronin 1a concentration was determined to be 85.97 ng/mL with a range of 4.85 – 716.8 ng/mL.

  • The concentrations of Coronin 1a were measured in duplicate and interpolated from the Coronin 1a standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Coronin 1a concentration was determined to be 21.4 pg/µg HEPG2 extract, 54.4 pg/ µg MCF7 extract and 888.5 pg /µg Jurkat extract.

  • PBMC cells were grown in the absence (unstimulated) or presence of Phorbol Myistrate Acetate (PMA and phytohemagglutinin (PHA) (stimulated) for 3 days. The concentrations of Coronin 1a were measured in duplicates, interpolated from the Coronin 1a standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated 50% and stimulated (25%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Coronin 1a concentration was determined to be 38.7 ng/mL in unstimulated and 76.9 ng/mL in stimulated, and 6.7ng/mL in media. This pair is highly cross reactive with bovine serum. Dilute bovine serum containing media such that the concentration of bovine serum is less than 0.1% or run a media control sample.

Protocols

References

ab214032 has not yet been referenced specifically in any publications.

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