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Our Abpromise guarantee covers the use of ab16381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Lane 1 : Hela whole cell lysate
Lane 2 : Human skeletal muscle cell lysate
Lane 3 : Hela whole cell lysate + COX IV blocking peptide (ab16381)
Lane 4 : Human skeletal muscle cell lysate + COX IV blocking peptide (ab16381)
Rabbit polyclonal to COX IV (ab16056) 0.38 µg/ml
Alexa fluor Goat anti Rabbit IgG 1/10000
All lysates loaded at 20µg per well
COX IV blocking peptide (ab16381) used at 1µg/ml
Observed band size : 15kDa
ab16381 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"