Overview

  • Product name

    Human CRISP3 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 18ng/ml 0.63 3.5%
    2 16 41ng/ml 1.7 4.1%
    3 16 72ng/ml 2.74 3.8%
    Inter-assay
    Sample n Mean SD CV%
    1 24 20ng/ml 1.14 5.7%
    2 24 38ng/ml 2.4 6.3%
    3 24 65ng/ml 3.8 5.8%
  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 50 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human CRISP3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213768) is designed for the quantitative measurement of Human CRISP3 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and tissue homogenates.


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CRISP3 amount of sample captured in plate.

  • Notes

    Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human CRISP3 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human CRISP3 antibody 1 x 130µl
    Lyophilized recombinant Human CRISP3 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Tissue specificity

    Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.
  • Sequence similarities

    Belongs to the CRISP family.
  • Cellular localization

    Secreted. In neutrophils, localized in specific granules.
  • Information by UniProt
  • Alternative names

    • Aeg 2
    • Aeg2
    • CRIS3_HUMAN
    • CRISP 3
    • CRISP-3
    • Crisp3
    • CRS 3
    • CRS3
    • Cysteine rich secretory protein 3
    • Cysteine-rich secretory protein 3
    • dJ442L6.3
    • MGC126588
    • OTTHUMP00000016590
    • OTTHUMP00000039911
    • SGP 28
    • SGP28
    • SGP28 protein
    • Specific granule protein (28 kDa)
    • Specific granule protein of 28 kDa
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human CRISP3 ELISA Kit (ab213768) Standard Curve.

Protocols

References

ab213768 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213768.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up