RecombinantSimpleStep

Human CRP ELISA Kit (C-Reactive protein) (ab260058)

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SimpleStep ELISA Protocol Diagram
  • Human CRP standard curve comparison
  • Human CRP ELISA kit comparison comparison
  • Example of human CRP standard curve in Sample Diluent NS.
  • Standard curve
  • Interpolated concentrations of native CRP in human serum and plasma samples.
  • Serum from ten individual healthy human male donors was measured in duplicate.
  • Linearity of dilution.
  • Linearity of dilution.
  • Recovery
  • Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (ab260058)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 5.36 pg/ml
  • Range: 18.75 pg/ml - 1200 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human CRP ELISA Kit (C-Reactive protein)
    See all C Reactive Protein kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 1.4%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    5.36 pg/ml

  • Range

    18.75 pg/ml - 1200 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100 99% - 100%
    Serum 109 105% - 112%
    Hep Plasma 114 112% - 119%
    EDTA Plasma 110 107% - 113%
    Cit plasma 107 105% - 109%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human CRP ELISA Kit (C-Reactive protein) (ab260058) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CRP (C-Reactive protein) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CRP (C-Reactive protein) with 5.36 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.


    ASSAY SPECIFICITY: This kit recognizes both native and recombinant human and monkey CRP protein in serum, plasma, and cell culture supernatant.


    Cell and tissue extract, urine, milk, and saliva samples have not been tested with this kit.


    CROSS REACTIVITY: Recombinant mouse CRP and rat CRP were both prepared at 50 ng/mL respectively and assayed for cross reactivity. No cross-reactivity was observed.


    INTERFERENCE: Recombinant mouse CRP and rat CRP were both prepared at 50 ng/mL respectively and tested for interference. No interference with was observed.


    SPECIES REACTIVITY:  This kit recognizes human and monkey CRP protein.


    Other species reactivity was determined by measuring 1:1000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.


    Reactivity < 3% was determined for the following species: Mouse, Rat, Cow.


    Other species reactivity not determined.

  • Notes

    C-reactive protein (CRP) displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6//interleukin-6. This kit requires the use of acid treatment for the disassociation of CRP.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Human CRP standard curve comparison
    Human CRP standard curve comparison

    Standard Curve comparison between human CRP SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Human CRP ELISA kit comparison comparison
    Human CRP ELISA kit comparison comparison

    Standard Curve comparison between ab260058 and ab181416 SimpleStep ELISA kits. ab260058 SimpleStep ELISA kit shows comparable sensitivity.

  • Example of human CRP standard curve in Sample Diluent NS.
    Example of human CRP standard curve in Sample Diluent NS.

    The CRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Standard curve
    Standard curve

    Example of human CRP standard curve in Sample Diluent NS. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native CRP in human serum and plasma samples.
    Interpolated concentrations of native CRP in human serum and plasma samples.

    The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1000, plasma (citrate) 1:2000, plasma (EDTA) 1:1000, plasma (heparin) 1:1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 1025.5 ng/mL in serum, 1676.5 ng/mL in plasma (citrate) and 668.3 ng/mL in plasma (EDTA), and, 1062.8 ng/mL in plasma (heparin).

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 535.7 ng/mL with a range of 28.1 – 1180.4 ng/mL.

  • Linearity of dilution.
    Linearity of dilution.

    Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

    Native CRP was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

  • Linearity of dilution.
    Linearity of dilution.

    Recombinant CRP was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

  • Recovery
    Recovery

    Three concentrations of CRP recombinant protein were spiked in duplicate to the indicated biological matrix to evaluate signal recovery in the working range of the assay.

  • Sandwich ELISA - Human CRP ELISA Kit&nbsp;(C-Reactive protein) (ab260058)
    Sandwich ELISA - Human CRP ELISA Kit (C-Reactive protein) (ab260058)
    To learn more about the advantages of recombinant antibodies see here.

References (0)

Publishing research using ab260058? Please let us know so that we can cite the reference in this datasheet.

ab260058 has not yet been referenced specifically in any publications.

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