Human CRP ELISA Kit (C-Reactive protein) (ab260058)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.36 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CRP ELISA Kit (C-Reactive protein)
See all C Reactive Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 1.4% Inter-assay Sample n Mean SD CV% Serum 3 4% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
5.36 pg/ml -
Range
18.75 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 100 99% - 100% Serum 109 105% - 112% Hep Plasma 114 112% - 119% EDTA Plasma 110 107% - 113% Cit plasma 107 105% - 109% Cerebral Spinal Fluid 88 80% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CRP ELISA Kit (C-Reactive protein) (ab260058) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CRP (C-Reactive protein) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CRP (C-Reactive protein) with 5.36 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY: This kit recognizes both native and recombinant human and monkey CRP protein in serum, plasma, and cell culture supernatant.
Cell and tissue extract, urine, milk, and saliva samples have not been tested with this kit.
CROSS REACTIVITY: Recombinant mouse CRP and rat CRP were both prepared at 50 ng/mL respectively and assayed for cross reactivity. No cross-reactivity was observed.
INTERFERENCE: Recombinant mouse CRP and rat CRP were both prepared at 50 ng/mL respectively and tested for interference. No interference with was observed.
SPECIES REACTIVITY: This kit recognizes human and monkey CRP protein.
Other species reactivity was determined by measuring 1:1000 serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow.
Other species reactivity not determined.
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Notes
C-reactive protein (CRP) displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. CRP can interact with DNA and histones and it may scavenge nuclear material released from damaged circulating cells. CRP is secreted; it forms a homopentamer pentaxin (or pentraxin) which have a discoid arrangement of 5 non-covalently bound subunits. CRP binds 2 calcium ions per subunit. The concentration of CRP in plasma increases greatly during acute phase response to tissue injury, infection or other inflammatory stimuli. It is induced by IL1/interleukin-1 and IL6//interleukin-6. This kit requires the use of acid treatment for the disassociation of CRP.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10 x 96 tests 10X Human CRP (C-Reactive Protein) Capture Antibody 1 x 600µl 1 x 6000µl 10X Human CRP (C-Reactive Protein) Detector Antibody 1 x 600µl 1 x 6000µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml Antibody Diluent 4BI 1 x 6ml 10 x 6ml Human CRP (C-Reactive Protein) Lyophilized Recombinant Protein 2 vials 2 x 10 vials Plate Seals 1 unit 1 x 10 units Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units Stop Solution 1 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 1 x 120ml -
Research areas
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Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
Tissue specificity
Found in plasma. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
Database links
- Entrez Gene: 1401 Human
- Omim: 123260 Human
- SwissProt: P02741 Human
- Unigene: 709456 Human
- Unigene: 76452 Human
Associated products
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Alternative Versions
Images
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Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples are 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Standard Curve comparison between human CRP SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Standard Curve comparison between ab260058 and ab181416 SimpleStep ELISA kits. ab260058 SimpleStep ELISA kit shows comparable sensitivity.
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The CRP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human CRP standard curve in Sample Diluent NS. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of CRP were measured in duplicates, interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1000, plasma (citrate) 1:2000, plasma (EDTA) 1:1000, plasma (heparin) 1:1000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 1025.5 ng/mL in serum, 1676.5 ng/mL in plasma (citrate) and 668.3 ng/mL in plasma (EDTA), and, 1062.8 ng/mL in plasma (heparin).
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CRP concentration was determined to be 535.7 ng/mL with a range of 28.1 – 1180.4 ng/mL.
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Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native CRP was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Recombinant CRP was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.
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Three concentrations of CRP recombinant protein were spiked in duplicate to the indicated biological matrix to evaluate signal recovery in the working range of the assay.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab260058 has been referenced in 11 publications.
- Yang Q et al. Clinical Effect of the Guizhi Shaoyao Zhimu Decoction in the Treatment of Hyperuricemia. Biomed Res Int 2022:5186210 (2022). PubMed: 36060146
- Zhang W et al. Downregulationof circ_0001578 promotes gestational diabetes mellitus by inducing placental inflammation via the NF-κB and JNKs pathways. Front Endocrinol (Lausanne) 13:657802 (2022). PubMed: 36263320
- Francis HM et al. Kynurenic acid as a biochemical factor underlying the association between Western-style diet and depression: A cross-sectional study. Front Nutr 9:945538 (2022). PubMed: 36299996
- Zhou Y et al. Detection Analysis of Perioperative Plasma and CSF Reveals Risk Biomarkers of Postoperative Delirium of Parkinson's Disease Patients Undergoing Deep Brain Stimulation of the Subthalamic Nuclei. Clin Interv Aging 17:1739-1749 (2022). PubMed: 36474580
- Kwon JH et al. Intravenous iron injection does not impair liver function in a rat model of cirrhosis. Ann Palliat Med 11:1687-1699 (2022). PubMed: 35016521