Human CT-1 ELISA Kit (ab216166)
- Datasheet
- References
- Protocols
Overview
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Product name
Human CT-1 ELISA Kit
See all CT-1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 3.2% Inter-assay Sample n Mean SD CV% Overall 5 7.2% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.2 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 115 104% - 122% Cell culture extracts 114 106% - 128% Tissue Extracts 109 102% - 121% Cell culture media 107 106% - 108% Hep Plasma 107 105% - 109% EDTA Plasma 109 106% - 116% Cit plasma 92 90% - 95% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
CT-1 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CT-1 protein in human serum, plasma, cell culture supernatant, and cell and tissue extract samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Samples in 1X Cell Extraction Buffer PTR - 3.2 pg/mL
Samples in Sample Diluent 25BS - 4.4 pg/mL
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Notes
CT-1 (Cardiotrophin 1) is a secreted member of the IL-6 cytokine superfamily. It is a 201 amino acid protein which binds to and activates the ILST/gp130 heterodimer receptor. CT-1 induces cardiomyocyte hypertrophy in vitro and is a biomarker implicated in hypertension. It is highly expressed in heart, skeletal muscle, prostate and ovary. It has lower expression levels in lung, kidney, pancreas, thymus, testis and small intestine. There is little or no expression of CT-1 in brain, placenta, liver, spleen, colon or peripheral blood leukocytes.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CT-1 Capture Antibody 1 x 600µl 10X Human CT-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human CT-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. -
Tissue specificity
Highly expressed in heart, skeletal muscle, prostate and ovary. Lower levels in lung, kidney, pancreas, thymus, testis and small intestine. Little or no expression in brain, placenta, liver, spleen, colon or peripheral blood leukocytes. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cardiophin 1
- Cardiophin1
- Cardiotrophin I
see all -
Database links
- Entrez Gene: 1489 Human
- Omim: 600435 Human
- SwissProt: Q16619 Human
- Unigene: 483811 Human
Associated products
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ELISA kits
Applications
Our Abpromise guarantee covers the use of ab216166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Human CT-1 ELISA Kit (ab216166)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Cardiotrophin 1 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Cardiotrophin 1 standard curve in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cardiotrophin 1 in human heart, skeletal muscle, and liver based on a 1,000 µg/mL extract load.The concentrations of Cardiotrophin 1 were measured in duplicate and interpolated from the Cardiotrophin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiotrophin 1 concentration was determined to be 1,029 pg/mL in heart, 1,550 pg/mL in skeletal muscle, and 78 pg/mL in liver.
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Interpolated concentrations of native Cardiotrophin 1 in PC-3 and U-87 MG cell extracts based on a 1,000 µg/mL extract load.The concentrations of Cardiotrophin 1 were measured in duplicate and interpolated from the Cardiotrophin 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cardiotrophin 1 concentration was determined to be 429 pg/mL in PC-3, and 811 pg/mL in U-87 MG.
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Interpolated concentrations of spike Cardiotrophin 1 in human plasma samples.The concentrations of Cardiotrophin 1 were measured in duplicates, interpolated from the Cardiotrophin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100%, plasma (heparin) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spike Cardiotrophin 1 in human serum and cell culture media (RPMI + 10% FBS) samples. concentrations of spike Cardiotrophin 1 in human plasma samples.The concentrations of Cardiotrophin 1 were measured in duplicates, interpolated from the Cardiotrophin 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% and cell culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Other species reactivity was determined by measuring a 1,000 µg/mL extract load of various species heart tissue extract.The protein concentrations were interpolated from the human Cardiotrophin standard curve.
Protocols
References
ab216166 has not yet been referenced specifically in any publications.
