Product nameHuman CXCL10 (IP10) knockout A549 cell lysate
See all IP10 kits
Knockout cell lysate achieved by CRISPR/Cas9.
Human CXCL10 knockout A549 cell lysate - Brefeldin A (5 µg/ml, 6h)
Human wild-type A549 cell lysate - Brefeldin A (5 µg/ml, 6h)
Human CXCL10 knockout A549 cell lysate - IFN-y (100 ng/ml, 32h), TNF-a (10 ng/ml, 32h) and Brefeldin A (5 µg/ml, during the last 6h of IFN-y/TNF-a treatment)
Human wild-type A549 cell lysate - IFN-y (100 ng/ml, 32h), TNF-a (10 ng/ml, 32h) and Brefeldin A (5 µg/ml, during the last 6h of IFN-y/TNF-a treatment)
Parental Cell LineA549
Mutation descriptionKnockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
Knockout validationSanger Sequencing, Western Blot (WB)
Reconstitution notesTo use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: After reconstitution, store the lysate at -80°C.
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
See here for more information on knockout cell lysates.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Storage instructionsStore at -80°C. Please refer to protocols.
Components 1 kit ab262033 - Human CXCL10 knockout A549 cell lysate - Brefeldin A treated (Lyophilized) 1 x 100µg ab277646 - Human CXCL10 knockout A549 cell lysate - IFN-y, TNF-&alpha; + Brefeldin A treated (Lyophilized) 1 x 100µg ab277645 - Human wild-type A549 cell lysate - Brefeldin A treated (Lyophilized) 1 x 100µg ab277647 - Human wild-type A549 cell lysate - IFN-y, TNF-α + Brefeldin A treated (Lyophilized) 1 x 100µg
STR AnalysisAmelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
FunctionChemotactic for monocytes and T-lymphocytes. Binds to CXCR3.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes.
- Information by UniProt
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
KO cell lines
Lane 1: Wild-type A549 Brefeldin A (ab120299)-treated (5ug/ml, 6h) cell lysate 30 ug
Lane 2: Wild-type A549 IFN-y (ab259377) (100 ng/ml, 32 h) and TNF-alpha (ab259410) (10 ng/ml) for 32 hours, and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate 30 ug
Lane 3: IP10 knockout A549 Brefeldin A (ab120299)-treated (5ug/ml, 6h) cell lysate 30 ug
Lane 4: IP10 knockout A549 IFN-y (ab259377) (100 ng/ml, 32 h) and TNF-alpha (ab259410) (10 ng/ml) for 32 hours, and Brefeldin A (ab120299)-treated (5ug/ml for the last 6h) cell lysate 30 ug
Lanes 1 - 4: Merged signal (red and green). Green - ab137018 observed at 11 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab137018 was shown to react with IP10 in wild-type A549 cells in western blot with loss of signal observed in IP10 knockout cell line ab266969 (IP10 knockout cell lysate ab256886). A549 wild-type and IP10 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab137018 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Homozygous: 1 bp insertion in exon2
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab256886 has not yet been referenced specifically in any publications.