Overview

  • Product name

    Human CXCL7 / PBP ELISA Kit
    See all CXCL7/PBP kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 8 3.1%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 3 4.9%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.51 pg/ml
  • Range

    4.69 pg/ml - 300 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 90 84% - 95%
    Heparin Plasma 88 84% - 90%
    EDTA Plasma 94 89% - 99%
    Citrate Plasma 94 92% - 96%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Product overview

    CXCL7 / PBP in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human CXCL7 / PBP protein in serum, plasma, and cell culture supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    CXCL7 / PBP is a platelet-derived growth factor that belongs to the CXC chemokine family. It is a potent chemoattractant and activator of neutrophils and has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, and prostaglandin E2 secretion. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Mouse and rat CXCL7 / PBP both have 66% sequence homology compared to human CXCL7 / PBP.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human CXCL7 Detector Antibody 1 x 600µl
    10X Human CXCL7 Capture Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BI 1 x 6ml
    Human CXCL7 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    LA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    Proteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)).
    Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor.
    NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils.
    NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • B TG1
    • Beta TG
    • Beta thromboglobulin
    • Beta-TG
    • C-X-C motif chemokine 7
    • Chemokine (C X C motif) ligand 7
    • Connective tissue activating peptide III
    • CTAP 3
    • CTAP III
    • CTAP-III
    • CTAP-III(1-81)
    • CTAP3
    • CTAPIII
    • CXC chemokine ligand 7
    • CXCL 7
    • CXCL7
    • CXCL7_HUMAN
    • LA PF 4
    • LA-PF4
    • LDGF
    • Leukocyte derived growth factor
    • Leukocyte-derived growth factor
    • Low-affinity platelet factor IV
    • Macrophage-derived growth factor
    • MDGF
    • NAP 2
    • NAP-2
    • NAP-2(1-63)
    • NAP-2(1-66)
    • NAP-2(73)
    • NAP-2(74)
    • Neutrophil activating peptide 2
    • Neutrophil-activating peptide 2(1-63)
    • PBP
    • Platelet basic protein
    • PPBP
    • Pro platelet basic protein
    • Pro platelet basic protein (chemokine (C-X-C motif) ligand 7)
    • SCYB7
    • Small inducible cytokine subfamily B member 7
    • Small-inducible cytokine B7
    • TC1
    • TC2
    • TGB
    • TGB1
    • THBGB
    • THBGB1
    • Thrombocidin 1
    • Thrombocidin 2
    • Thromboglobulin, beta-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab216171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of CXCL7 were measured in duplicates, interpolated from the CXCL7 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:4,000, plasma (citrate) 1:8,000, plasma (EDTA) 1:3,000, and plasma (heparin) 1:3,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL7 concentration was determined to be 1,106 ng/mL in neat serum, 1,434 ng/mL in neat plasma (citrate), 636 ng/mL in neat plasma (EDTA), and 604 ng/mL in neat plasma (heparin).

  • The concentrations of CXCL7 were measured in duplicates, interpolated from the CXCL7 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 1:64. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL7 concentration was determined to be 15.61 ng/mL in neat PBMC supernatant.

  • PBMC media samples were cultured in RPMI media with 10% Fetal Bovine Serum for 48 hours without (unstimulated) or with (stimulated) 1.5% PHA-M.

  • Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL7 concentration was determined to be 873 ng/mL with a range of 335 – 2800 ng/mL in neat human serum.

Protocols

References

ab216171 has not yet been referenced specifically in any publications.

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