Overview

  • Product name

    Human CXCL9 ELISA Kit
    See all CXCL9 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    8.23 pg/ml - 6000 pg/ml
  • Recovery

    97 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101.3 89% - 108%
    Serum 94.56 82% - 102%
    Plasma 96.32 84% - 103%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s CXCL9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL9 in serum, plasma, and cell culture supernatants.


    This assay employs an antibody specific for Human CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human CXCL9 (lyophilized) 2 vials
    CXCL9 Microplate (12 x 8 wells) 1 unit
    Recombinant Human CXCL9 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-X-C motif chemokine 9
    • chemokine (C-X-C motif) ligand 9
    • CMK
    • crg-10
    • CXCL9
    • CXCL9_HUMAN
    • gamma interferon induced monokine
    • Gamma-interferon-induced monokine
    • HuMIG
    • MIG
    • monokine induced by gamma interferon
    • monokine induced by interferon gamma
    • Monokine induced by interferon-gamma
    • SCYB9
    • Small inducible cytokine B9
    • small inducible cytokine subfamily B member 9
    • Small-inducible cytokine B9
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100595.

  • Representative Standard Curve using ab100595.

Protocols

References

This product has been referenced in:

  • Ren X  et al. Effects of fluconazole on the clinical outcome and immune response in fungal co-infected tuberculosis patients. Microb Pathog 117:148-152 (2018). ELISA . Read more (PubMed: 29432913) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

MIG-CXCL9 human macrophage secretion

Excellent Excellent 5/5 (Ease of Use)
Abreviews
MIG-CXCL9 secretion by human macrophages isolated by negative selection in culture for 3 & 7 days.
Kit easy to use, the assay takes about 6hr, good specificity as shown by higher secretion by M1 macrophages (MSCF+IFNg) compared to the others subtypes (M0=MCSF M2=MSCF+IL4 DEX=MCSF+Dexamethasone IL10=MCSF+IL10 TGFb=MCSF+TGFb)

Abcam user community

Verified customer

Submitted Apr 01 2014

Answer

Following up my e-mail from yesterday, the lab has requested some information that will help us assess the issue.

In addition to the raw data, could you please tell us:

At what temperature were the kits stored and for how long?

How did you prepare/dilute the HRP-streptavidin?

Do you feel the issue is with the standard only because the samples showed strong ODs?

Finally, have you tried the 2nd fresh/un-reconstituted vial of standard yet?

I look forward to your reply.

Read More

Answer

Thank you for contacting us and bringing this to our attention. For our records, can you please tell me again the test you described that indicated the antibodies in these kits are likely not the issue? Have you tested any samples other than the standards?

Assuming you followed the instructions for preparing the standards, I expect we will simply send out another two kits free-of-charge. Did you deviate at all from the preparation protocol? I am concerned that some of the standard may not have gone into solution. Did you notice any turbidity or undissolved material when you reconstituted the lyophilized standards?

I appreciate your help with this, and hope we can get the assay working correctly for you.

Read More

Answer

Thank you for your call last week and for your patience while I have been intouch with the lab regarding your enquiry.

I've heard back from the lab, and there is no BSA or anything else in the wash buffer that we would expect to produce higher background. Was there anything else altered in the protocol? Would you be able to send data for us to take a look at?

Please let me know if you have any other questions or if there is anything else that we can do for you, and I'll be happy to help.

Read More

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