Key features and details
- Sensitivity: 20 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman CXCL9 ELISA Kit
See all CXCL9 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 20 pg/ml
Range8.23 pg/ml - 6000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 101.3 89% - 108% Serum 94.56 82% - 102% Plasma 96.32 84% - 103%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s CXCL9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL9 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CXCL9 (lyophilized) 2 vials CXCL9 Microplate (12 x 8 wells) 1 unit Recombinant Human CXCL9 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionCytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
- Information by UniProt
- C-X-C motif chemokine 9
- chemokine (C-X-C motif) ligand 9
ab100595 has been referenced in 1 publication.
- Ren X et al. Effects of fluconazole on the clinical outcome and immune response in fungal co-infected tuberculosis patients. Microb Pathog 117:148-152 (2018). ELISA . PubMed: 29432913